FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9393285
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACC9750.030.7084123
TGTCGGC31050.027.15235130
TCGTGAC11550.025.654982
CGCGAAG12600.024.2620331
CGAGCAG17550.022.9289571
CGGCGGC36300.022.82115433
GTGGGAC37750.022.51277419
CGGGAAG75500.022.1457041
CGGCAAG23900.022.0577141
TCGGCGG39800.020.99846632
GAAGCGT44250.020.9609569
GTGACCT14900.020.3033144
AATGTCG44000.019.7440828
CGCGGAG6350.019.6497921
GGGTCTC46600.019.35235861
TGAAGCG48000.019.324168
AAGCGTA49650.019.05052470
GAATGTC46100.018.84467527
TAGTCCG51850.018.22678611
GTCCGCA52050.018.09663413
Produced by FastQC (version 0.11.5)