FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11565401
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG18350.025.6184161
CGGCAAG36400.025.2308581
CGGCAAT38800.024.5528031
CGCGGAG9300.024.4371931
CGGCAAA81550.024.432471
CGGGAAG66150.023.1080361
CGCGAAA30050.022.8959751
CGGGCTG7500.022.8303621
CGGGAAA57900.022.3679491
CGCGAAT18350.021.8859311
CGGGGAG11900.021.7141361
CGCCAAA52650.021.4644451
CGGCAAC34700.021.3529971
CGGGCAG14300.021.335431
CGGGAAT27100.021.1378081
CGCCAAG35200.020.430581
CGCGCAT6700.019.9804381
CGGGGTG11450.019.8485491
CGCGCAG7600.019.6625161
CGGGCTT12850.019.139711
Produced by FastQC (version 0.11.5)