FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9942952
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151920.1527916457808506No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG101270.10185103981191904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG5900.025.5624141
CGCCGTA105300.024.64098753
CCGTATC101250.024.47133655
GTCGCCG111700.023.91634851
TCGCCGT108900.023.62519852
TGTCGGC27250.023.60048530
GGTCGCC114900.023.5047250
CGTATCA107450.023.3312656
GCCGTAT117800.023.11208554
GTCGGCG28550.022.52651631
TGGTCGC120050.022.13239349
CGGCGGC29850.021.79016933
CGCGAAG13550.021.1015681
TCGGCGG31000.020.74607532
GTGGTCG132650.020.52579548
CTCGGTG108700.020.30699744
CGGCAAG23550.020.1464541
CGGGAAG91150.020.096691
GTATCAT116050.019.64999857
TCTCGGT113400.019.23986843
Produced by FastQC (version 0.11.5)