FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7968872
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG123710.1552415448510153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC96900.027.30802555
CGCCGTA99150.026.65205253
GTCGCCG111600.026.49610751
TCGCCGT100050.026.41288252
GGTCGCC112150.026.30117850
CGTATCA103600.026.0356856
GCCGTAT113550.025.81519754
TGGTCGC111000.025.45538349
CTCGGTG109650.024.76689744
GTGGTCG122100.024.09906648
ATCTCGG115900.023.77700442
TCTCGGT114000.023.69342443
CGGGCAG8450.023.4176141
GATCTCG114500.023.36480141
GTATCAT112150.022.7472357
GGTGGTC130150.022.35556647
TCGGTGG138450.022.09732845
CGCGCAG3800.021.49061
AGATCTC124500.021.48811140
TGTCGGC17150.020.66991830
Produced by FastQC (version 0.11.5)