FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16638357
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG285670.17169363537517557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA273600.030.5351553
GGTCGCC302500.030.09015350
GTCGCCG303350.030.0291551
CCGTATC270050.029.91911755
TCGCCGT276300.029.87901352
CGTATCA281500.029.44370556
GCCGTAT305250.029.3381754
TGGTCGC300600.029.0145149
CTCGGTG283250.028.21624644
GTGGTCG324350.027.94051648
ATCTCGG293200.027.58171342
TCTCGGT286650.027.51064543
GATCTCG289050.027.38103341
GGTGGTC340100.026.47419247
TCGGTGG349750.026.2565945
AGATCTC304350.025.99233640
CGGTGGT370850.025.08930446
GTATCAT315300.024.98594757
TAGATCT343100.024.39131739
TCGCCGG36150.022.9079452
Produced by FastQC (version 0.11.5)