FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13474771
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218430.16210294037650064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC236250.027.68836255
CGCCGTA247850.027.47086353
TCGCCGT250600.027.14090252
CGTATCA242900.027.14071756
GTCGCCG261750.026.72597951
GGTCGCC265750.026.47557450
GCCGTAT273450.026.31794754
TGGTCGC272050.025.55464249
CTCGGTG232750.025.35431944
ATCTCGG240600.024.82945342
TCTCGGT239950.024.71547343
GATCTCG230600.024.61976241
GTGGTCG286450.024.48694448
GTATCAT260800.023.649957
AGATCTC249200.023.28148540
GGTGGTC299200.023.18671847
TCGGTGG289700.023.0999945
CGGTGGT316650.022.04777346
CATTAAA307000.021.4385761
TAGATCT288700.021.33845539
Produced by FastQC (version 0.11.5)