FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19738308
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG71600.025.6783771
CGCGAAG35500.024.6828861
CGGCAAA136350.024.1498131
CGGGAAG98150.023.0237711
CGCGAAA54350.022.8539471
CGGCAAT74500.022.3264881
CGCGAAT30150.021.9245431
CGGGAAT49950.021.7894951
CGGGAAA97000.021.0146051
CGGGCAG28550.020.8917581
CGCCAAA96350.020.5500811
CGCCAAG59850.020.548271
CGGGCTG15300.020.2959481
CGGGCAA36400.020.0182631
CGGGCTA17100.019.9575061
CGCGGAG20400.019.7433381
CGACAAA119850.019.5733831
CGGCAAC63050.019.2615591
CGCGCAG16450.019.2508871
CGAGCAG50400.018.6668191