FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13256371
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG18800.026.7411841
CGGCAAG36750.025.4054241
CGGCAAT42800.023.7840861
CGGGAAT30100.023.2377531
CGCGAAA32850.022.8133181
CGGGGTG13850.022.7711121
CGGGGAG13550.022.5839211
CGCGCAG8550.022.2780081
CGGCAAA93950.022.1355381
CGGGAAG75700.022.0271341
CGGGAAA64400.021.9646841
CGAGCAG29900.020.8867781
CGGGCTG7050.020.3742351
CGACAAG49350.019.9313181
CGCGAAT20600.019.857141
CGGGCAG14100.019.48841
CGCGGAG10100.019.4774361
CGCCAAA59500.019.417681
CGCCAAT38150.019.3984221
CGGGCTA9750.019.2158361
Produced by FastQC (version 0.11.5)