FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4380127
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA55590.12691412828897428No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48390.11047624874803859No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC13350.031.87069930
ACTAGCC5750.029.7909362
TCGGCGG15200.027.26850932
GTGGGAC16600.026.79073519
CACTAGC6700.026.0331841
GAATGTC16800.025.98080327
CGAGCAG5100.024.42891
CGGCAAG6950.024.2004281
CGCGAAA4300.023.9033951
GGCGGCA17500.023.68496734
AATGTCG18600.023.66372928
CTAGCCA7300.023.4611783
TGTCGTG16000.023.1654681
GAAGCGT23400.022.89028269
CGGGAAG30400.022.847851
GTCGGCG18900.022.124631
GCCCGGC9600.022.05781615
CGGCAGT18800.021.65754336
CCCGGCG9850.021.49797416
TGAAGCG25000.021.279768
Produced by FastQC (version 0.11.5)