FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10507686
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208160.198102607938608No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA107420.10222992959629741No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC105930.10081191996030334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC184500.030.1641455
CGTATCA188300.029.84557756
CGCCGTA200300.028.89451853
TCGCCGT206000.027.95362752
GCCGTAT217450.027.83961754
GTATCAT187750.027.79770957
CGAGATC34800.027.7973025
TCATTAA199200.027.69986760
GTCGCCG215950.027.3915151
GGTCGCC219350.027.3495450
CATTAAA207100.027.2418661
TGGTCGC226800.026.11497349
ATCATTA210550.025.98192459
TGTCGGC39600.025.12266530
CTCGGTG203050.025.02243244
TATCATT221300.024.65393858
TCTCGGT206550.024.51019543
ATCTCGG208150.024.39049542
GATCTCG198650.024.34550341
CGGCAAG25400.024.0246121
Produced by FastQC (version 0.11.5)