FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12372169
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1758461.4213029259461296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAG12500.019.9128461
CGCCGTA22100.019.7759153
CCGTATC20800.019.60414755
CGCGAAG5150.019.5775811
TCGCCGT22800.019.00100552
CGGGAAT4450.017.7009371
CGTATCA23950.017.63771856
GCCGTAT27400.017.41303354
CTCGGTG23300.016.73486744
GATCTCG24150.016.27438741
TCTCGGT24600.015.99757743
CGCGAAA5800.015.2105991
CGGAGGT5000.015.1820253
GTATCAT26500.014.154201557
CGGGAAA8550.014.00340751
TCGGTGG32950.013.93514645
AGATCTC29900.013.87528540
CGCACAG2306.6023513E-613.6989871
CGCCAAG5350.013.5453531
TAGTCCG6850.013.44838111
Produced by FastQC (version 0.11.5)