FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n01_vk3-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n01_vk3-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8362236
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT10450.029.13851225
CGGGCTG10700.029.11190626
ACACTCT40700.029.0706141
CGCCGGG10400.028.94206623
AGGCCGC11650.026.4373916
CCGCCGG11750.025.91467922
GGCCGCC12350.025.50570717
GCCGGGC12500.024.63979524
GAGGCCG13150.023.68787215
GGGCTGC13400.022.98488227
CCTACGA14550.019.2437136
GCCCACA28550.018.5141071
CGCCGCC18200.016.92283420
TCCTACG18300.016.83035935
CGAGGCC18150.016.77661914
CACTCTT94800.016.6902181
TCGAGGC19750.015.59471313
CCGCCGC20000.015.57477619
CTACGAC19200.014.58312537
GGCTGCA22850.014.39804328
Produced by FastQC (version 0.11.5)