FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n01_vk3-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n01_vk3-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6542073
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCACTCTTCTTCTGAAGAGTTAAGCACTCCATTATGCTCATTGGGTTG81290.12425724995731476No Hit
TTTCTTGGCTTCCTATGCTAAATCCCATAACTAACCTACCATTCGATTCA69520.10626601078893494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGATCG41500.032.55916
CGGGCTG5700.030.08717226
ACGTCTG127750.030.08164222
CCTTGTA129150.029.15958240
ACCTTGT130050.028.84991539
GATCGGA134250.028.834628
AGATCGG134100.028.7885747
CGCCGGG6100.028.68800223
TGCCGTC45150.028.6041355
CACACGT135500.028.54191419
GTATGCC53800.028.42906652
TCCAGTC133650.028.3870632
AGTCACC133200.028.35158735
CGTCTGA136550.028.2199223
CAGTCAC133900.028.17722934
GTCTGAA136200.028.1639524
GAGCACA138350.028.02984616
CCAGTCA135100.027.90104533
TATGCCG51450.027.75480853
TCGGAAG140950.027.48881110
Produced by FastQC (version 0.11.5)