FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n01_vk3-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n01_vk3-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9393285
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT94100.041.927471
CGCCGGG13950.036.6303723
GGTCACG10100.036.03915812
CCGGGCT14100.035.49601425
GCCGGGC14750.034.88092424
CGGGCTG15250.033.50778226
GGCCGCC16500.032.02990317
AGGCCGC16650.031.11072716
GGGCTGC16650.030.69031127
GAGGCCG18300.029.26193215
CCGCCGG18650.027.39912422
TCGAGGC20850.026.18672613
CCTACGA18300.024.28931636
TCCTACG20550.022.99241635
AGGTCAC16450.022.55315411
CACTCTT190300.022.2960641
ATCGAGG24600.022.19484512
GGCTGCA24900.021.08407628
CTACGAC21950.021.04757737
GTCACGA18700.020.21365213
Produced by FastQC (version 0.11.5)