FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n01_vk3-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n01_vk3-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9942952
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTG11300.044.60111626
CCGGGCT11950.042.46825
CGCCGGG12950.039.72894723
GGCCGCC15200.034.0782817
AGGCCGC16000.032.81185516
GGGCTGC16000.032.37452727
CCGCCGG16400.031.15792522
GAGGCCG17900.030.11114115
GCCGGGC17150.029.79548324
CCTACGA17900.026.9827136
TCGAGGC19850.026.80048213
CGCCGCC19550.026.13759420
CCGCCGC20350.025.7980219
GCCGTCT114300.024.19061756
TCCTACG21450.023.33287635
CTGCTTG103850.023.15313164
TATGCCG146700.022.37890453
TGCCGTC138550.022.3564555
ATCGAGG25150.021.9876412
GCTTGAA116550.021.95151966
Produced by FastQC (version 0.11.5)