FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n01_vk3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n01_vk3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7968872
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGGG10100.036.73258223
CGGGCTG10650.035.8215126
CCGGGCT11150.033.27346425
GGCCGCC12000.031.7913917
CCGCCGG12650.030.4347122
GGGCTGC13000.029.34608327
CGCCGCC14400.026.97893120
AGGCCGC14200.026.86596516
GAGGCCG14650.026.27963615
TCGAGGC15000.025.66644113
GCCGGGC14750.025.15248124
CCGCCGC16150.023.62208719
CGAGGCC16800.023.3331314
TCCTACG15550.022.95799835
CCTACGA15150.022.87108836
ACACTCT37350.020.7121831
TAGGCAT212750.020.61321642
ACTTAGG233350.020.4883639
CTTAGGC234800.020.27239640
TTAGGCA232100.020.17647241