FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n01_vk3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n01_vk3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16638357
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGG175350.025.14941246
TGGCGGG43000.024.82529855
GAGATCG176700.024.6236556
GCGTGAA59450.024.49081466
GTATCGG184600.024.4580145
CCGATGT585250.024.10644540
CGATGTA584300.024.04380841
ATCGGGG108550.023.69848447
TGTATCG348900.023.59388444
ATCGGGT78300.023.46716347
ACCGATG606050.023.34839439
AAGATCG281500.023.327726
TGCCGGC58250.023.07269955
ATGTATC573350.023.03184743
GATGTAT592900.022.98077842
CACCGAT620150.022.8401138
GTCACCG620800.022.77673336
TGCCGGG39500.022.7718955
TATCGCG152750.022.4776546
CAGTCAC634400.022.4043134
Produced by FastQC (version 0.11.5)