FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n01_vk3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n01_vk3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13474771
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT10000.029.04970225
GCTTGAA289950.027.37685466
CTGCTTG270800.027.27076564
CGGGCTG10850.026.12876326
TGCTTGA285250.026.0856265
GCCGTCT327400.025.9985156
CCGTCTT345400.024.9476357
TGCCGTC370850.024.80222755
TATGCCG387800.024.30489553
ACACTCT72450.024.3044071
ATGCCGT384350.024.05864354
GTATGCC406100.023.46820352
TCTGCTT330550.022.77544263
CGTATGC419550.022.68248751
GCGTGAA40150.022.49042766
GATCTCG370150.022.11648446
CTTGAAA369400.022.10453867
TCGTATG422600.021.99701550
CTCGTAT394400.021.98134649
GTCTTCT359600.021.73364659
Produced by FastQC (version 0.11.5)