FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n01_vk3-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n01_vk3-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13256371
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT10850.017.74169325
CGGGCTG11550.017.27249126
GCCGGGC11400.017.19274724
CGCCGGG11850.016.83521323
ACACTCT70650.016.4504621
AGGCCGC13550.015.23964416
GGCCGCC14100.014.39696717
CCGCCGG15150.013.630177522
GGGCTGC15750.013.11093227
ACGACGC113000.012.63718610
CCGCCGC15850.012.58657919
CCTACAC119650.012.4627255
GAGGCCG16650.012.40223315
GACGCTC111800.012.39696812
CGACGCT113500.012.3348211
CCCTACA120950.012.2128264
CTACACG124800.012.060546
ACACGAC123800.012.0154568
CACGACG131500.011.7377489
CGCCGCC17250.011.56505920