FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n01_vk3-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n01_vk3-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4380127
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG88730.20257403495378104TruSeq Adapter, Index 9 (100% over 39bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA74040.16903619461262198Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC69410.15846572485227026TruSeq Adapter, Index 9 (100% over 37bp)
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG61080.13944801143893773Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT57790.13193681370426016TruSeq Adapter, Index 9 (100% over 36bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA56740.12953962293787372TruSeq Adapter, Index 9 (100% over 38bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT56000.12785017420727754Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC55150.12590959120591708TruSeq Adapter, Index 9 (100% over 37bp)
ACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA55070.12572694809990667TruSeq Adapter, Index 9 (100% over 38bp)
ACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT55060.12570411771165538Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC46350.10581884954477347Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA45310.10344448916663831TruSeq Adapter, Index 9 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT120450.058.2783931
CCGGGCT13400.052.23797625
CGGGCTG13450.051.7835726
CGCCGGG14100.049.644623
GGGCTGC14050.049.57217427
GCCGGGC14350.048.77971624
TCGAGGC15500.047.87021313
AGGCCGC15100.047.7475916
GAGGCCG15600.047.1146415
GGCCGCC15400.046.5901717
GGCTGCA16650.041.83117328
CCTACGA12300.041.8286136
TCCTACG13150.041.7864135
CCGCCGG17050.041.4656222
ATCGAGG18200.040.9608912
CCCATGA19150.040.9523931
TATCGAG19450.039.22817211
CCATGAA21850.035.7267842
CTACGAC12550.035.41776737
TGCATCC19500.034.8199631
Produced by FastQC (version 0.11.5)