FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n01_vk3-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n01_vk3-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10507686
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGATCG107200.027.8851876
GCGGGGT48250.027.63698257
TGGCGGG38950.027.22702655
GGGTGTA30700.026.79121466
GGGTGGA25050.026.68631666
CCGGGCT9400.026.06352225
CGGGGTG51950.024.86015158
AATCGGG196250.024.70034846
ATCGGGG156700.024.50194547
CGGGGTT80800.024.3438949
TCGGGGT86700.023.73685848
TTGCGGG41250.023.58771555
CGGGCTG10100.023.5640826
GAATCGG211900.023.43766645
CGGCGTG22700.023.12748158
ACACTCT63850.023.0262721
GTGTGGG35300.022.80426662
TGCGGGG36150.022.46180756
CGGGGGT52100.022.43746449
ATCGGGT44350.022.41236347
Produced by FastQC (version 0.11.5)