FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12372169
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG952980.7702610593178932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT40350.019.0541531
CCGGGCT2951.3249519E-714.21965825
GAGATCG32250.013.86899956
CGGGCTG3405.1471943E-813.36615926
GCCCTGA4751.7644197E-1012.5073651
GCCCACA10700.012.4111011
GCGCGAA2050.003387751511.9331391
GGCGTGC2356.942431E-411.8968681
GCCCTCG4105.898837E-711.0807711
AAGATCG55000.011.0548126
ACTTAGG7650.010.97527139
GACCTGA5905.693437E-1010.6618111
GCGAGAT7301.8189894E-1210.5307524
GGGCTGC4005.2480264E-610.48856627
AGTCACT21450.010.44152835
AGTCACC29650.010.38650335
CACCGAT18300.010.32309338
ACGTCTG113950.010.30707722
CCCTACA55000.010.1630859
GGGCATG3106.096827E-410.1459181
Produced by FastQC (version 0.11.5)