FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13397492
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138750410.356445818366602No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN206750.15431992793875152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAAG101600.036.1890753
TCTATGC26800.031.5979253
GAGCAAA129250.030.4838031
AGCAGGG65400.029.9580768
GAGCGAA17200.029.8972131
CTATGCT29700.029.4779994
CTCTATG48900.027.6846892
ACAAGGG28800.027.1418083
GCAGGTA65650.025.7645269
CACCGTC16250.024.918147
GCAGGGT45900.024.8820089
AGCAAAA167450.023.3893932
CACTGTG32550.023.102737
CAAGGGT37550.022.9301074
ACAAGGT27800.022.911073
GGTAGTT24100.022.802247
GCACCGT18500.022.6722266
CGAAAGC32950.022.6250363
AAGGTAG26400.022.466435
AGGACAG40600.022.0912275
Produced by FastQC (version 0.11.5)