FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_INFANT_Exp2_Shed_Ind2_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_INFANT_Exp2_Shed_Ind2_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361010
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC49450.36333311290879566No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA39390.28941741794696585No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT39220.2881683455668952No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT37410.27486939846143676No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA37320.27420812484845813No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA30350.22299615726556013No Hit
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG28030.20594999301988964No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA24130.17729480312415044No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA23880.1754579319769877No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT23340.17149029029911608No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA22230.16333458240571339No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA19980.1468027420812485No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA18640.13695711273245603No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG17230.12659715946245803No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT17220.1265236846165715No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC16880.12402553985643015No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT16750.12307036685990551No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG16730.12292341716813249No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG16480.12108654602096972No Hit
GTAGTAAACAGTATTTGCAACACTACAGGAGATATGCTTATAAGAAGTGC15840.11638415588423304No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG15750.11572288227125443No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC15160.11138786636395029No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT15080.11080006759685822No Hit
CTACAGGAGATATGCTTATAAGAAGTGCCATAGGCCAGGTTTCAAGGCCC14920.10962447006267403No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA14720.10815497314494384No Hit
TCTCTGTACTGAGAACGTAGGTTGTATGCTGATTTGGCCCGCAGATGCCC14680.1078610737613978No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT14660.10771412406962476No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA14290.10499555477182386No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT14260.10477513023416432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG100.0070708334145.13914145
ACGCCCG100.0070708334145.13914145
GAGCGAA2150.0104.438971
AGCAGGC7750.096.20638
AGTACCG259.351773E-487.083496145
GCTGTCG353.421405E-582.93665145
TTTAAAC1700.072.398873
TAAACTA1700.072.396225
GCAAAAG30950.072.047993
AGCGAAA3850.067.730011
GCAGGGT8700.066.563899
GAGCAAA37300.061.1703641
AGAGTTA851.29602995E-859.76318145
CAAAAGC38150.058.827854
AAGCAGG43550.056.684737
TTTTAAA2350.052.3775022
ACAAGGG4300.052.1945343
CTTCCCG701.6867085E-551.83541145
CGATACA858.868319E-751.225582145
TTATATA1750.049.6431124
Produced by FastQC (version 0.11.5)