FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_INFANT_Exp2_Shed_Cnt4_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_INFANT_Exp2_Shed_Cnt4_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765516
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC61460.348113526017323No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA43240.24491423470532125No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA38520.21817984090770062No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA37900.21466811968852165No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA29040.1644844906531575No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA23970.1357676735866455No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT22890.129650481785495No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC19340.10954304577245406No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT19070.10801374782216644No Hit
AATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCA18950.10733405984426082No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT18110.10257624399892155No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA17890.10133014937276127No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC17720.10036725807072834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG20050.050.566615
TACTGAT19200.049.784977
ACTGATC23650.046.534658
AAGGTCG3950.045.8215759
AGCGAAA6250.042.892471
GTACTGA27350.041.570016
GGCGACC907.446842E-540.312508145
CTATTCG5950.040.1535649
TAACACG1105.3063486E-639.5021744
AGCAGGT24900.038.991041
TCGCCAT6450.037.125004145
CGAAAGC7300.034.7232173
TATGGAT22150.034.0101242
CTGATCC32400.033.2941869
GTATGGA26850.033.190991
TCGTACT2206.2937033E-1032.9250072
AAGAGCG8650.032.6502386
AACACGT1352.159649E-532.1860475
CAGGTAC31600.031.8568213
GAGCGGT9000.031.3751748
Produced by FastQC (version 0.11.5)