FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_INFANT_Exp2_NL_Cnt4_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_INFANT_Exp2_NL_Cnt4_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492763
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC53420.21430035667249553No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC52010.2086439826008329No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG51850.20800212455014777No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT47390.19011033138729996No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC46410.18617895082685357No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT45780.1836516347522809No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA43560.1747458542990248No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT42930.17221853822445213No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA42140.16904936409919435No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT40020.16054474492761647No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA39020.15653313211083444No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT37870.15191977737153511No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA37470.15031513224482232No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG35010.14044656471553854No Hit
GTCTAGTAGAATAAGCATCTATTGGACAATAGTTAAGCCGGGAGACGTAC34510.13844075830714753No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA33470.13426868097769423No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT32870.13186171328762503No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT32430.13009660364824094No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT31910.1280105649835143No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA30050.1205489651442997No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA29980.12026815224712498No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA29800.11954606194010421No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT29080.11665770071202115No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA29000.1163367716866786No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT27800.11152283630654017No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA27700.11112167502486196No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA26160.10494379128701767No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC25880.1038205396983187No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT25420.10197519780259896No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT25380.10181473328992768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3050.083.1229551
AGCGAAA6450.072.997231
AGTACCG809.822543E-1172.56979145
GCAGGGT20100.062.3001379
GGCAGCC4250.061.47088145
AGCAGGC3900.059.3926968
CCGACGC1007.1486284E-1058.05583145
CGAAAGC8200.056.5045623
GCAAAAG54250.054.7143823
GACGTCT400.006086733754.296264
ACAAGGG9600.052.789093
GGCGACC553.204675E-452.77803145
CCGTATA701.6870885E-551.835564145
CAAAAGC58150.051.292774
AAGGGTG8350.050.2833335
TGGGCCC1750.049.762142145
CAAGGGT10300.049.2004974
GGCTAGC901.3156241E-648.37986145
GTACCCG752.5651036E-548.2904781
AGACGTC450.00969539148.2643133
Produced by FastQC (version 0.11.5)