FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_HBEpC_LOW_3DPI_PR8_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_HBEpC_LOW_3DPI_PR8_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436533
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA70840.4931317275690847No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG69500.4838037135241585No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG47420.33010031791821004No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG41730.2904910642498293No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT39630.2758725347764374No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT34030.2368897895140592No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA31910.2221320359504446No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG31460.21899949392043205No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT30700.21370897849196643No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT29430.20486824876281992No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG28680.1996473453794657No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA28650.19943850924413153No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA27780.1933822613194406No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT27730.19303420109388367No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA27050.18830058202630917No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT26720.18600338453763332No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT25350.17646653435737292No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA25300.17611847413181597No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT24960.1737516645980287No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG24570.17103679483868453No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA24230.1686699853048973No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA23460.16330985783132027No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT22480.1564878774104041No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC21970.1529376631097232No Hit
CAATACTATTTGAGGCAAATGGAAATCTAATAGCACCAATGTATGCTTTC21490.1495962849443765No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA20670.14388809724524254No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC20590.14333120088435142No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA20420.14214779611745781No Hit
CCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAAC20140.14019865885433888No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC20090.13985059862878194No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA19640.1367180565987694No Hit
GTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAGAT19120.13309823025297712No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG19100.13295900616275436No Hit
TGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATG18910.13163637730563793No Hit
GTACGGGGACGGAGCTTCACCGACAGGGCAGCTCATTAAGGCCCTATAAG18810.13094025685452404No Hit
CTGCAGATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCA18790.13080103276430127No Hit
GCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGTACAATTCAAG18650.12982646413274182No Hit
GTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATGGG18650.12982646413274182No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG18560.1291999557267393No Hit
GTCTGGAACTGTGACTTTTGGTCCTTCCTATCCAAACACCATTACCATAC18420.12822538709517983No Hit
ATACTGGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGG18290.1273204305087318No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA18130.12620663778694954No Hit
ATATTATTGTGTCTCCGGGTTTTAGCAAGGTCCAGTAATAGTTCATCCTC17980.1251624571102787No Hit
GTCTCTGTCCATCCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGA17940.12488400892983315No Hit
GTTCCAGACATGGGTTTGAGATGATTTGGGATCCTAATGGATGGACAGAG17880.12446633665916482No Hit
GCTATACCCTGACCAATCAGTCATTGCCACAACATCTTGCCTAACAGAGA17590.1224475873509345No Hit
CATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGGAACAG17520.12196030303515477No Hit
GGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGG17420.12126418258404088No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT17340.12070728622314976No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA17260.12015038986225865No Hit
GGCCATCAGTCATTATAGTAAAACATGAACCATTTACACAGGCACATTCA16620.1156952189751297No Hit
GCCACTGCTCCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCAT16530.1150687105691272No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA16450.11451181420823607No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT16420.1143029780729019No Hit
CACCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCA16370.11395491784734496No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT16210.11284112512556274No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA16050.11172733240378048No Hit
ATCAAATAGGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCC15990.11130966013311215No Hit
CCATTGGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAA15650.1089428505993249No Hit
TCCCCGTACAATTCAAGATTTGAATCGGTTGCTTGGTCAGCAAGTGCATG15580.10845556628354518No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA15510.10796828196776545No Hit
CCATCAACATACACTGGACCACAGCTGCCTGTTCCATCTTCGGGACGCGG15390.10713293742642878No Hit
AACATACACTGGACCACAGCTGCCTGTTCCATCTTCGGGACGCGGGTTGT15370.10699371333620598No Hit
CTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCT15180.10567108447908959No Hit
GAATATAATCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAA15170.1056014724339782No Hit
TGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCAATCTACA15080.1049749640279757No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC14720.10246893040396565No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG151.2241851E-4145.17601145
ACGGCCG900.088.71868145
TGACGGC2450.085.678082
AATACTA4650.084.0579152
GCAAAAG16150.080.666353
TACTATT5000.079.604894
CGGCCCG2750.078.9475
AGCAAAA17400.076.959162
GACGGCC2800.074.960493
ACGGCCC2800.074.952654
GTACCCG300.001944031872.423561
AGCGAAA3550.071.403521
CCCCACG1400.067.40315145
ACTATTT6050.066.985345
GCCTAGT1200.066.388271
CTAGTAT1200.066.344343
GCACACC2450.065.18107145
GGGCTTA451.1868868E-464.522675145
GAGCGAA1700.059.642941
GCAGGGG10050.057.6004149
Produced by FastQC (version 0.11.5)