FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_HBEpC_LOW_1DPI_PR8_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_HBEpC_LOW_1DPI_PR8_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732522
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA79240.4573679295270132No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG71700.41384755864572No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT52410.3025069811523317No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT50650.2923483799917115No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA49560.2860569735911001No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG47000.27128082644837986No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA42590.24582660422205316No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG38840.22418185743095903No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT37570.21685150318437513No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA37560.2167937838595989No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT37450.21615887128706013No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT36760.2121762378774988No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA35620.2055962348530062No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG33890.19561079166671475No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA33200.19162815825715343No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC31650.18268166291683452No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA30700.1771983270630907No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT29580.17073376268815058No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG29190.1684827090218768No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG29050.16767463847500927No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA27900.1610369161257404No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG27730.1600556876045441No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT27700.15988252963021538No Hit
GAGTTATGGGCTGGCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGG27660.15965165233111037No Hit
GTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGCCTGACTAGCAAC27340.15780463393827035No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT26720.15422603580214278No Hit
GCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAG26510.1530139299818415No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT25610.1478191907519789No Hit
GACTTGAAGATGTCTTTGCAGGGAAGAACACTGATCTTGAGGTTCTCATG25490.14712655885466389No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA24850.14343252206898383No Hit
CTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTATC24600.14198953894957755No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA24430.14100831042838127No Hit
GAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAGACCAA24400.14083515245405254No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT23870.13777602824091123No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG22960.13252356968627238No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA22890.13211953441283864No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA22870.13200409576328612No Hit
CCTTTCAGTCCGTATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACG22690.13096514791731362No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG22490.1298107614217886No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC22450.12957988412268356No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG22430.12946444547313107No Hit
CTGCAGATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCA22400.1292912874988023No Hit
GCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGTACAATTCAAG22280.1285986556014873No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA22050.12727111113163353No Hit
GGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCA21920.12652075990954229No Hit
GTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATGGG21680.12513549611491223No Hit
CCATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTT21470.12392339029461098No Hit
CCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAAC21420.12363479367072973No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT21270.12276900379908597No Hit
GTCAAGTGCAAGATCCCAATGATATTTGCGGCAATAGCGAGAGGATCACT21120.12190321392744219No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC20980.12109514338057469No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT20900.12063338878236465No Hit
CAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGTATGTG20900.12063338878236465No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT20830.12022935350893091No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA20700.11947900228683965No Hit
GTCTGGAACTGTGACTTTTGGTCCTTCCTATCCAAACACCATTACCATAC20540.11855549309041963No Hit
GTTCCAGACATGGGTTTGAGATGATTTGGGATCCTAATGGATGGACAGAG20520.11844005444086714No Hit
CACCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCA20480.11820917714176213No Hit
GTTCACAGGTTGCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCC20390.11768970321877587No Hit
CCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGC20180.1164775973984746No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG19980.11532321090294956No Hit
GCTATACCCTGACCAATCAGTCATTGCCACAACATCTTGCCTAACAGAGA19950.11515005292862081No Hit
TGTCTAGCCTGACTAGCAACCTCCATGGCCTCTGCTGCTTGCTCACTCGA19660.11347619251010953No Hit
GCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGGGATTTTAGGAT19580.11301443791189954No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC19580.11301443791189954No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG19490.11249496398891326No Hit
GAGTGATGGGCTGGCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGG19460.11232180601458451No Hit
CATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGGAACAG19440.11220636736503202No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA19120.11035934897219199No Hit
GGCTAGACAAATGGTGCAAGCGATGAGAACCATTGGGACTCATCCTAGCT19120.11035934897219199No Hit
GTCTCTGTCCATCCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGA19050.10995531369875822No Hit
GCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCCTGTT19050.10995531369875822No Hit
GGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGG19040.10989759437398197No Hit
GGCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGGGATTTTAGGA18930.10926268180144322No Hit
GAATGTGCCTGTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCC18860.10885864652800945No Hit
GTACGGGGACGGAGCTTCACCGACAGGGCAGCTCATTAAGGCCCTATAAG18760.10828145328024695No Hit
ATACTGGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGG18580.10724250543427442No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCTCATATACAACA18570.10718478610949816No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG18550.10706934745994567No Hit
GTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAGAT18520.10689618948561692No Hit
GATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCATGGCC18300.1056263643405394No Hit
CACTTGAACCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGCCTGCAA18170.10487601311844814No Hit
ATCCTGTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATA18140.1047028551441194No Hit
TGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATG18020.10401022324680437No Hit
AACATACACTGGACCACAGCTGCCTGTTCCATCTTCGGGACGCGGGTTGT17970.10372162662292313No Hit
ATCAAATAGGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCC17840.10297127540083185No Hit
GTAGAAGGCCCTCCTTTCAGTCCGTATTTAAAGCGACGGTAAATGCATTT17640.10181688890530682No Hit
TACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCCTGTTGTATA17550.10129741498232057No Hit
GTTTTAGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAG17490.10095109903366306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCCG554.129106E-1092.35325145
AGCGTAC406.0876664E-790.70409145
CCTAGCG259.355438E-487.07593145
GAGCGAA1600.086.004561
GCAAAAG17950.083.885323
AGCAAAA20050.074.7555542
GCGTTCA300.001929294272.56326145
GCAGGGG8700.069.868949
TTAGTAG651.4087345E-766.853751
AGCAGGG14350.065.055958
CTGTCTA902.8012437E-1064.50068145
AAGCAGG26400.062.7813847
GCAGGGT5100.062.4319089
AGCGAAA2800.062.0784761
ACACATA1500.058.050613145
ATGCCGA502.0209834E-457.9399151
TTACTTG502.0261841E-457.9097984
CTACTAA753.8073085E-757.9014366
CCCCACG1400.057.013992145
GGCTAGC1153.8198777E-1156.788643145
Produced by FastQC (version 0.11.5)