FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_HBEpC_LOW_1DPI_H3N2_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_HBEpC_LOW_1DPI_H3N2_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831047
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG82500.45056189163904586No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG67980.3712629987105738No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC55060.30070227580176806No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC53120.2901072446529226No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA50160.2739416301165399No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT49840.2721939961126066No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG46940.2563560629519614No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA41830.22844853245165198No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA40550.22145799643591893No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT34030.18584995360577855No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG34000.1856861129179098No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG33780.1844846145402057No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT33000.18022475665561832No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG32340.17662026152250598No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC32000.17476340039332688No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG31570.17241501720054156No Hit
CTCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCC30330.16564293543530012No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC30000.16384068786874395No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG29960.1636222336182523No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT29510.16116462330022113No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA29120.15903469435792747No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT28580.15608556197629006No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG27370.14947732089891738No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT26310.14368828326088845No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA25960.14177680856908645No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA25860.14123067294285727No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA25330.1383361541238428No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC23510.12839648572647236No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT23480.1282326450386036No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA22780.12440969565499958No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT22700.12397278715401625No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT22580.12331742440254129No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA22370.12217053958746008No Hit
GCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGGT22180.12113288189762468No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT22030.12031367845828098No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA21770.1188937258300852No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC21580.11785606814024982No Hit
TCCTAGCTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA21530.11758300032713524No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG20780.11348698313041665No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG20700.11305007462943331No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC20650.11277700681631875No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGG20560.11228548475271252No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCA20450.11168473556386045No Hit
CTCCCAGCACAGGTCTCATAGGCAAATGGTGGCAACAACCAACCCATTAA20450.11168473556386045No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA20360.11119321350025423No Hit
TCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCT20180.11021016937304176No Hit
TACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA20120.10988248799730428No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC19800.10813485399337101No Hit
CTCATTGCCTGCACCATTCGCCTGGCCTGACTAGCAATTTCCATGGCCTC19660.1073702641166502No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT19610.10709719630353563No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT19470.10633260642681482No Hit
GTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCCATTGAGGGCA19370.10578647080058567No Hit
CTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGGTTG18850.10294656554419412No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA18670.10196352141698165No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT18500.1010350908523921No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT18450.10076202303927753No Hit
CCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATG18410.10054356878878587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG256.4396318E-6116.09653145
CGTAGTG203.8929947E-4108.5645
TCACGCG259.3570293E-487.072395145
AGGCTCG1150.075.715126145
GTCAAGG702.7957867E-972.56033145
CAGTACG1450.069.8707359
GCAGGGT6250.060.208619
TAGAAAC11000.057.9087072
CGGGCTA400.00603180354.42025145
ACGGATA1750.053.76653
AGGGTGT7100.051.9869736
TAAACTA2150.050.4948845
GGTCGTT1152.5247573E-950.347157
AGGTCGT1152.5247573E-950.347156
GCAGGGG5550.049.547979
AGCGTAC450.00960877448.373554145
AGACGCG450.00960877448.373554145
GGCTTTA604.925917E-448.37355145
TACGGAT1950.048.2572632
CCACTCG450.00970058148.257262
Produced by FastQC (version 0.11.5)