FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_HBEpC_LOW_1DPI_H1N1_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_HBEpC_LOW_1DPI_H1N1_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040512
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA43550.41854394759503016No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT41460.39845768237175544No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA34090.32762716816336573No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC33930.32608946364866526No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT33250.31955421946118834No Hit
GGGCTAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC33050.31763208881781274No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT32550.3128267622093739No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG30110.28937676836019194No Hit
CCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCTTCTTGTATAGTTTA28230.27130874031246155No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG28190.27092431418378643No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC28080.2698671423299299No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAT26200.25179911428219953No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC25340.24353395251568458No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGCAG25100.2412273957436339No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGATAAAGC24770.2380558801820642No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC24300.2335388731701316No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACGTTCC23260.2235437938245787No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT21980.21124215770697502No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG21950.2109538381104687No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG21300.2047069135194981No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC21190.20364974166564154No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC21130.20307310247262889No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA20950.20134318489359085No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACTTGGAGAAAAGAATAGAGA20870.2005743326362406No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA20780.19970937384672163No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC20350.19557679296346417No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT19630.1886571226473121No Hit
TTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC19090.18346736991019805No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCTGCTGC18960.18221798499200395No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC18930.1819296653954976No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG18490.17770097798007137No Hit
GAACTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT18100.173952823225489No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC17990.17289565137163243No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC17950.1725112252429573No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA17490.1680903247631935No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC17380.16703315290933693No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC17290.16616819411981795No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG17210.1653993418624677No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT17160.16491880920162383No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC16980.1631888916225858No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT16380.1574224996924591No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT16310.15674975396727764No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAG16050.15425098413088942No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT16000.15377045147004553No Hit
GCACTGGAACTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG15820.1520405338910075No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC15810.15194442735883873No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT15750.15136778816582605No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT15560.14954176405461928No Hit
GCCTAGTCTGATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT15530.14925344445811292No Hit
ATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGTGT15530.14925344445811292No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA15260.1466585680895559No Hit
GATTAGTGGATTGGTGGTAGCAGCCATTTGTCTGTGAGACCGATGCTGTG15190.14598582236437446No Hit
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC14870.14291041333497356No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC14520.13954668470906628No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA14330.1377206605978595No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA14330.1377206605978595No Hit
ATCCAGCCACCTGTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATT14280.13724012793701562No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCAGAGGCCATGG14140.1358946364866527No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT13860.13320365358592692No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG13850.13310754705375816No Hit
GGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAAT13800.13262701439291424No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC13690.13156984253905768No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT13680.1314737360068889No Hit
GATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGCCACC13650.1311854164103826No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTCTCAG13620.13089709681387626No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC13600.1307048837495387No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT12870.12368910690121786No Hit
CTATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGT12850.12349689383688031No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC12850.12349689383688031No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC12840.12340078730471153No Hit
AGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACA12790.12292025464386765No Hit
CTTGTATAGTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCAT12740.12243972198302375No Hit
GACTAGGCAGATGGTACATGCAATGAGAACTATTGGGACTCATCCTAGTT12730.12234361545085497No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT12550.12061369787181694No Hit
GCTTAGTGACACCTCCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCT12530.12042148480747938No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT12530.12042148480747938No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG12440.1195565260179604No Hit
GGTACATGCAATGAGAACTATTGGGACTCATCCTAGTTCCAGTGCTGGTC12440.1195565260179604No Hit
TTCCCATTTAGGGCATTTTGGATAAAGCGTCTACGCTGCAGTCCTCGTTC12190.11715386271374092No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC12160.11686554311723459No Hit
CTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCT12140.11667333005289703No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC12040.11571226473120926No Hit
TCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGCCACCTGTTCCATAGC12030.11561615819904048No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG11980.11513562553819659No Hit
GCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGTGTCACTAAGCT11890.11427066674867757No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG11890.11427066674867757No Hit
TCTCAGACCAGCACTGGAACTAGGATGAGTCCCAATAGTTCTCATTGCAT11870.11407845368434003No Hit
ATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAGGATCA11830.1136940275556649No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACC11820.11359792102349613No Hit
GACTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG11800.11340570795915857No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG11800.11340570795915857No Hit
CTTGAAACGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAAT11680.11225242957313322No Hit
ATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGCAGCCATTTGTCT11670.11215632304096444No Hit
CTTTTAAGCTTCTTGTATAGTTTAACTGCTCTATCCATGTTGTTCGGGTC11650.11196410997662688No Hit
GTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATACATTT11650.11196410997662688No Hit
ATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGCTCCTG11630.11177189691228935No Hit
GGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACT11550.11100304465493911No Hit
CTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTCTCAGACCAGCAC11540.11090693812277033No Hit
CAGTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATG11390.10946534014023865No Hit
TATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCCA11260.10821595522204452No Hit
GACCAGCACTGGAACTAGGATGAGTCCCAATAGTTCTCATTGCATGTACC11220.10783152909336943No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG11180.10744710296469431No Hit
ATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGT11120.10687046377168163No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT11100.10667825070734407No Hit
CAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCT11030.10600550498216263No Hit
ATGTAGGATTGGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA10870.10446780046746217No Hit
AAATGGGAATGGGGACCCGAACAACATGGATAGAGCAGTTAAACTATACA10780.10360284167794317No Hit
CAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTG10730.10312230901709928No Hit
GATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACTT10690.10273788288842417No Hit
TCCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCTTCTTGTATAGTTT10520.10110407184155493No Hit
GTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAG10490.10081575224504859No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG10450.10043132611637347No Hit
ATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGA10450.10043132611637347No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACCG253.69364E-8145.11703145
TCCGGTA100.007119772144.803073
ATACACG100.0071207955144.796114
GAGCGAA1700.0102.263111
TCTGACC750.096.74469145
GGATTAA850.093.899254145
TCGGTCG259.356811E-487.07022145
GCGTTTA353.42361E-582.92402145
GCAGGTA8750.081.8974849
AGGCTCG900.080.620575145
GAGCAAA13600.078.295191
CGCGAGC300.001929577472.55852145
AGTAGAA4300.070.75181
CTAGACT955.456968E-1268.587644
AACTGCG350.003555160262.193012145
CGTGTAA350.003555160262.193012145
ATCGGTA702.3216307E-762.193012145
GACTCCG350.003555160262.193012145
TACGTCT350.003555160262.193012145
GTAGAAA4950.061.434562
Produced by FastQC (version 0.11.5)