FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_HBEpC_LOW_12HPI_PR8_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_HBEpC_LOW_12HPI_PR8_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380108
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA52340.379245682221971No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT51010.369608755256835No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG47920.3472192031348271No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA43680.3164969698023633No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT43490.315120265950201No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT39160.28374590974039715No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA36830.2668631730270385No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA33290.24121300651833047No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG32870.23816976642407695No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA31670.22947479472620985No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT29610.21454842664487125No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC28460.2062157454344153No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA28300.20505641587469964No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG27380.19839027090633485No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG25450.18440585809226523No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT24950.18078295321815394No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG24590.1781744617087938No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT24070.17440664063971806No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA24020.1740443501523069No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG23570.17078373576560676No Hit
GCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAG23100.1673782051839421No Hit
GTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGCCTGACTAGCAAC22660.1641900488947242No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT22570.16353792601738415No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG22080.15998747924075507No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA21950.15904552397348612No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA20830.15093021705547682No Hit
CCTTTCAGTCCGTATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACG20670.1497708874957612No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG20670.1497708874957612No Hit
GTCAAGTGCAAGATCCCAATGATATTTGCGGCAATAGCGAGAGGATCACT20500.14853909983856337No Hit
CTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTATC20480.1483941836435989No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT20410.14788697696122333No Hit
GAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAGACCAA20100.14564077593927433No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA19940.1444814463795587No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA19910.14426407208711203No Hit
TGTCTAGCCTGACTAGCAACCTCCATGGCCTCTGCTGCTTGCTCACTCGA19550.14165558057775188No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT19450.14093099960292962No Hit
GACTTGAAGATGTCTTTGCAGGGAAGAACACTGATCTTGAGGTTCTCATG19330.14006150243314291No Hit
GGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCA19030.13788775950867613No Hit
GTTCACAGGTTGCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCC18930.13716317853385387No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT18860.1366559718514783No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT18580.13462714512197596No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT18410.1333953574647781No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG18080.13100424024786464No Hit
CAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGTATGTG18060.1308593240529002No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC17350.12571479913166217No Hit
CCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAAC17300.12535250864425104No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA17200.12462792766942878No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT17050.12354105620719538No Hit
TACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCCTGTTGTATA16810.12180206186762196No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG16680.12086010660035303No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC16680.12086010660035303No Hit
GGCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGGGATTTTAGGA16660.12071519040538856No Hit
GGCTAGACAAATGGTGCAAGCGATGAGAACCATTGGGACTCATCCTAGCT16610.12035289991797744No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA16540.11984569323560185No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG16320.11825161509099288No Hit
GCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGGGATTTTAGGAT16300.11810669889602843No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAAGCA16250.1177444084086173No Hit
GTAGAAGGCCCTCCTTTCAGTCCGTATTTAAAGCGACGGTAAATGCATTT16230.11759949221365285No Hit
CCATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTT16120.11680245314134836No Hit
GTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCATTAAGGGCA16000.11593295597156164No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG15950.11557066548415051No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCTCATATACAACA15940.11549820738666829No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG15910.1152808330942216No Hit
GAGTTATGGGCTGGCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGG15870.1149910007042927No Hit
GTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATGGG15840.11477362641184603No Hit
ATCCTGTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATA15710.1138316711445771No Hit
GCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCCTGTT15690.11368675494961263No Hit
CACTTGAACCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGCCTGCAA15620.11317954826723706No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA15580.11288971587730814No Hit
CCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGC15530.11252742538989703No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTACTCTCTATCATCCCGTCAGGC15470.11209267680500368No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC15290.1107884310503236No Hit
GTTTTAGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAG15280.11071597295284137No Hit
GTACGGGGACGGAGCTTCACCGACAGGGCAGCTCATTAAGGCCCTATAAG14930.10817993954096346No Hit
CACCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCA14830.10745535856614119No Hit
CTGCAGATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCA14810.10731044237117676No Hit
GTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAGAT14800.10723798427369452No Hit
GTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCACCATTTGCCTATGAG14770.10702060998124784No Hit
TGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATG14730.10673077759131894No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA14580.10564390612908556No Hit
GTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCTCATATACAACAGG14570.10557144803160332No Hit
GGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTACT14510.10513669944670996No Hit
GTTCCAGACATGGGTTTGAGATGATTTGGGATCCTAATGGATGGACAGAG14480.1049193251542633No Hit
CATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGGAACAG14460.10477440895929883No Hit
GACTAAGGGGATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAGTGAGC14430.10455703466685216No Hit
GTGTGTGCAGAGACAACTGGCATGGTTCGAACCGGCCATGGGTGTCTTTC14400.10433966037440548No Hit
GAATAACTGAGTGAGATTTCTTTGGCCCCATGGAATGTTATCTCCCTCTT14080.10202100125497424No Hit
CTGCACACACACATCACTTTGCCGGTATCAGGGTAACAGGAACATTCCTC14080.10202100125497424No Hit
GTCTCTGTCCATCCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGA13980.10129642028015198No Hit
GTCTGGAACTGTGACTTTTGGTCCTTCCTATCCAAACACCATTACCATAC13920.10086167169525861No Hit
GTTGTATGGGCCTCATATACAACAGGATGGGGGCTGTGACCACTGAAGTG13880.10057183930532972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1700.0106.523131
AGCAAAA17350.087.2410662
GCAAAAG17700.085.0975343
CGCACTT451.242599E-680.4520655
TCACGCA300.001929898472.55675145
AGCGAAA3700.066.562571
GCAGGGT5150.066.0656749
AAGCAGG27050.062.0967567
CTGTCTA954.5292836E-1061.100418145
AGCAGGG14700.060.084058
GCAGGGG9100.059.6630029
CCTAGAG502.0193445E-457.948591
CAAAAGC26800.056.2004364
CCTCGCG400.00603266854.41756145
GCTACCC400.00603266854.41756145
CGCCGTT400.00603266854.41756145
GACACGC400.00607263954.3267971
AGGGTGT2950.053.9944156
AAGGTCG553.2399292E-452.659535
AAGGGTG3050.052.2278945
Produced by FastQC (version 0.11.5)