FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_HBEpC_LOW_12HPI_H1N1_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_HBEpC_LOW_12HPI_H1N1_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417510
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT42960.30306664503248654No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC37400.2638429358523044No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA33880.2390106595367934No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC33560.23675317987174693No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT31930.22525414282791653No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT31340.22109191469548714No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA30860.2177056951979175No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT30570.2156598542514691No Hit
GGGCTAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC29950.2112859874004416No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG28390.20028077403334016No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG25730.1815154743176415No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT25610.18066891944324906No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC24790.17488412780156753No Hit
CCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCTTCTTGTATAGTTTA24690.17417866540624052No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG23500.165783662901849No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG23410.1651487467460547No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC23150.16331454451820446No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACTTGGAGAAAAGAATAGAGA21390.15089840636044896No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACGTTCC21270.15005185148605654No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC21130.14906420413259872No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAT20370.1437026899281134No Hit
GGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAAAGTC20090.14172739522119773No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC19250.13580151110045077No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC19220.1355898723818527No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG19000.13403785511213326No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC18630.1314276442494233No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA18630.1314276442494233No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT18570.13100436681222707No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT18530.1307221818540963No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA18270.128887979626246No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC18180.1282530634704517No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC18140.1279708785123209No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA17790.12550176012867634No Hit
GAACTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT17460.12317373422409718No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTG17360.12246827182877017No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC17260.12176280943344314No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC16310.1150609166778365No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT16210.11435545428250947No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCTGCTGC16140.11386163060578057No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC16090.11350889940811705No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGATAAAGC16000.11287398325232273No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGCAG15760.11118087350353789No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC15720.11089868854540709No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA15690.11068704982680898No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC15520.10948776375475305No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT15480.10920557879662224No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC15340.10821793144316443No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT15280.1077946540059682No Hit
TTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC15090.10645427545484687No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA14840.10469061946652934No Hit
ATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGTGT14820.10454952698746393No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC14810.10447898074793123No Hit
GCACTGGAACTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG14700.10370297211307151No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG14690.10363242587353881No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCAGAGGCCATGG14680.10356187963400611No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAG14560.10271532475961367No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA14500.10229204732241748No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT14500.10229204732241748No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC14490.10222150108288477No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT14260.10059893757363263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2100.089.6464841
AGCGAGC2100.062.201324145
CGAAAGC4950.059.945773
GCGAAAG5150.057.6238822
GCAGGTA9300.055.2392779
TCACGCG400.006028929354.426163145
GAGCAAA22150.054.2642171
ATATGCG1402.7830538E-1046.5225755
ACAAGGG10250.045.8954243
GTAGAAA7750.045.7797661
AGTAGAA6650.045.73061
AGCAAAA27250.043.5615432
AGGCTCG2000.043.54093145
TATGCGT1505.456968E-1043.4210666
ACCGTCT1851.8189894E-1243.0222938
ATGCGTC1557.548806E-1042.0189067
TGCGTCT1557.548806E-1042.0129748
GCAGGGT12550.040.9342889
GATCCGT2150.040.391696
CCGAGCC907.5724354E-540.1975989
Produced by FastQC (version 0.11.5)