FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_HBEpC_HIGH_6HPI_PR8_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_HBEpC_HIGH_6HPI_PR8_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557793
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT38910.24977644654970207No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG38000.24393484885347413No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA37510.24078937317088983No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA35090.22525457490180015No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT34790.22332877346348326No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC32470.20843590900716588No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC31950.2050978531807499No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA31530.2024017311671063No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT30610.19649594008960114No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG30380.19501949232022484No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT29270.18789402699845228No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT27690.17775147275664996No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA25510.16375731563821383No Hit
ATATAATGCAGAATTGTTAGTTCTACTGGAAAATGAAAGGACTCTGGATT23950.15374314815896592No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA22680.1455905887367577No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA22280.1430228534856685No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG22000.14122543880990607No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA21860.14032673147202485No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG21550.1383367366524307No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT21280.1366035153579455No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC21260.13647512859539104No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA21130.13564061463878704No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC20400.13095449780554927No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA20350.1306335308991631No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA20330.13050514413660866No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA20250.12999159708639083No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT19980.1282583757919056No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA19120.12273774500206382No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT18630.11959226931947955No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT18040.11580485982412299No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG17680.1134938980981427No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA17490.11227422385387531No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG17370.11150390327854856No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT17360.11143970989727134No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT17310.11111874299088519No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG17280.1109261628470535No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA17190.11034842241555841No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA17090.10970648860278612No Hit
GCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAG16900.10848681435851874No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG16560.10630423939509293No Hit
GTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGCCTGACTAGCAAC16310.10469940486316218No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT16220.10412166443166712No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG15830.10161812256185512No Hit
CAATAGATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTG15690.10071941522397392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCCG700.0103.63927145
GAGCGAA1950.096.6087651
GTTGACG259.363348E-487.056984145
GCAAAAG18650.081.156263
AGCGAAA4100.075.991041
AGCAAAA21050.072.944762
CTGTCTA2000.072.547485145
GCAGGGG7950.066.485459
GCAGGGT11600.065.539369
GGCAGCC2550.065.43499145
AGCAGGG21700.060.3953748
TAGGCCG502.0040962E-458.03799145
TTAGTAG651.0978181E-555.7358251
GGTCCAC1206.002665E-1154.410618145
TCGACTT400.006035858854.410618145
GCTCACG553.209168E-452.761806145
ACAAGGG5850.051.9866947
AAGCAGG32750.051.7372447
AATACTA1600.049.7946972
CAAAAGC31400.049.35114
Produced by FastQC (version 0.11.5)