FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_HBEpC_HIGH_6HPI_H3N2_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_HBEpC_HIGH_6HPI_H3N2_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727989
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC85990.4976304826014517No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT64410.37274542835631475No Hit
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG51880.30023339268942106No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA51520.2981500460940434No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG48320.27963140969068667No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC46390.2684623571099122No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG45990.26614752755949256No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA45860.2653952079556062No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT40630.23512881158387003No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA39770.2301519280504679No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT39370.22783709850004832No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC38610.2234389223542511No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG35860.20752446919511638No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT35530.20561473481602024No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC32820.1899317646119275No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC31020.17951503163503935No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA30730.17783678021098512No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA29850.17274415520006206No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC29740.17210757707369664No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC29410.17019784269460048No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT28620.16562605433252178No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT28460.16470012251235394No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC26790.15503570913935216No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC26330.15237365515636964No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG24370.14103099035931363No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA23890.13825319489881013No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT23700.1371536508623608No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC23570.13640133125847445No Hit
GTTCTTATCTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAA23430.13559114091582758No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG23350.13512817500574367No Hit
CCCTAAGAGGAAGAGGCAATACTCTCGGTCTAGACATCAAAGCAGCCACC23250.1345494676181388No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT22350.12934110112969469No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA22260.1288202644808503No Hit
CTCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCC22090.12783646192192197No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA22040.12754710822811952No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG21910.12679478862423316No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT21790.12610033975910726No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG21590.12494292498389746No Hit
GTCTAGACATCAAAGCAGCCACCCATGTTGGAAAGCAAATTGTAGAAAAG21470.12424847611877159No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA21460.12419060538001109No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA21050.12181790509083101No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT20940.12118132696446564No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT20020.11585721899850057No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT19840.11481554570081175No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC19800.1145840627457698No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT19690.1139474846194044No Hit
CTATTGAGGAATTGTCAAGAAACTGGTTCATGCTAATGCCCAAGCAGAAA19680.11388961388064392No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG19660.11377387240312294No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT19450.11255858688915266No Hit
CTCCACACCTGCTTCGCGATACATAACTGACATGACTATTGAGGAATTGT19400.1122692331953502No Hit
ACTAGAGACCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTG19320.11180626728526628No Hit
ACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGCTA19240.11134330137518238No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA19110.110590981771296No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG18730.10839189369839738No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG18710.10827615222087641No Hit
CTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT18370.10630854710301976No Hit
CAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATT18110.10480390789524702No Hit
GTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAACATGATGTT18060.10451455420144456No Hit
ACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTCACCAT17810.10306778573243233No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT17640.10208398317350399No Hit
GTTTTTAGTCTGTGTCTCACTTCTTCAATCAGCCATCTTATCTCTTCAAA17620.10196824169598302No Hit
ACCAAGAACTGAGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAG17450.10098443913705468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG203.854171E-4108.83761145
AGGCTCG1550.079.58018145
GCACGGG300.001929806372.5584145
CGGTCTG754.827598E-967.721176145
CGAAAGC7450.067.059683
AGCGAAA7350.067.0121841
CGGGCTA451.189354E-464.49636145
CCTAGCG808.039933E-963.4886145
TCGACGC350.003555581762.192913145
GCAGGGT15250.058.8565449
TAGAAAC11400.058.4422762
AGGGTGT9750.057.173126
ACGTAAC400.00608309254.3037643
GCAGGGG5600.054.2880449
GCGAAAG9500.052.598052
CGGCTCG701.6884376E-551.827427145
GCTTATA2800.051.726862
AGCAGGG24200.051.147418
GTCAAGG1004.676258E-850.79088145
TAAACTA1152.5156623E-950.3657955
Produced by FastQC (version 0.11.5)