FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_HBEpC_HIGH_1DPI_H1N1_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_HBEpC_HIGH_1DPI_H1N1_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522487
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT58180.38213790988034707No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA57740.3792479016241189No Hit
GGGCTAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC49730.3266366149596023No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC47570.3124493017017551No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA46360.3045017789971277No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACTTGGAGAAAAGAATAGAGA43500.2857167253316449No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC41670.27369691826596876No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT39480.2593125589906515No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG37230.24453410768039396No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT36930.24256364750569298No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC36090.23704635901653018No Hit
CCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCTTCTTGTATAGTTTA34570.22706269413137847No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC32910.2161594811646996No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG31550.20722672837272174No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC31020.2037455820640833No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACGTTCC30740.20190648590102905No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGCAG29280.19231691305081752No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG29260.1921855490391708No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC28500.1871937165965949No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC27480.1804941520026115No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC27200.17865505583955724No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC26500.1740573154319216No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC26040.17103594316404672No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT25790.16939389301846255No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT25520.16762047886123166No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA25210.1655843366807073No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA24870.16335114848271284No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT24740.16249728240700906No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG24270.15941022813331082No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC24200.15895045409254727No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC23940.15724272194113972No Hit
GCACTGGAACTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG23920.157111357929493No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG23700.15566635380137894No Hit
GAACTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT23570.15481248772567516No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAT23140.1519881614752704No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCTGCTGC23090.15165975144615357No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG22980.15093724938209654No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC22140.14541996089293374No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA21920.14397495676481967No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT21790.1431210906891159No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT21740.14279268065999906No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT21730.1427269986541757No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC21420.14069085647365134No Hit
ATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG21410.14062517446782796No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT20880.13714402815918955No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC20820.13674993612424932No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT20680.13583038804272218No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGATAAAGC20550.13497652196701843No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT20520.13477947594954834No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20310.13340015382725764No Hit
ATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGTGT20280.13320310780978753No Hit
GCCTAGTCTGATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT20230.1328746977806707No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT20060.13175810368167348No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC19920.13083855560014634No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA19810.1301160535360893No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA19790.1299846895244426No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA19700.12939355147203227No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC19520.12821127536721166No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC19000.12479581106439662No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT19000.12479581106439662No Hit
CTATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGT18890.12407330900033957No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC18480.12138034676158155No Hit
TTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC18110.11895011254611698No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCAGAGGCCATGG18100.11888443054029361No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC18090.11881874853447025No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGG17650.11592874027824211No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAG17640.11586305827241875No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT17120.11244759396960369No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG16950.11133099987060645No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC16810.11041145178907932No Hit
TCTCAGACCAGCACTGGAACTAGGATGAGTCCCAATAGTTCTCATTGCAT16790.11028008777743258No Hit
GATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGCCACC16770.11014872376578584No Hit
GGGTAAAGAGTTCAACCACTTGGAGAAAAGAATAGAGAATTTAAATAAAA16750.11001735975413912No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA16590.10896644766096525No Hit
GCTTAGTGACACCTCCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCT16480.10824394559690821No Hit
GATTAGTGGATTGGTGGTAGCAGCCATTTGTCTGTGAGACCGATGCTGTG16240.10666757745714742No Hit
ATGTAGGATTGGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA16140.10601075739891375No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG15990.10502552731156325No Hit
TCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGCCACCTGTTCCATAGC15870.10423734324168285No Hit
CAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCT15590.10239824707862859No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG15420.10128165297963135No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA15400.10115028896798461No Hit
AAATGGGAATGGGGACCCGAACAACATGGATAGAGCAGTTAAACTATACA15290.10042778690392759No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA15260.10023074088645748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2500.0124.555621
AGGCTCG1300.0111.662605145
GGATTAA700.0103.68671145
AGCGAGC702.910383E-1182.94937145
GCAGGTA13150.082.5468759
GAGCAAA26400.074.0618741
TGCTACG607.9728125E-872.58069145
CGAAAGC4600.069.237944
ACACCCC955.456968E-1268.76066145
GTAGAAA8050.067.468371
GCGAAAG4950.062.8841323
CTGTAAG350.003589806162.042355
GCAGGGT8750.055.411759
AGCAAAA35400.054.604532
TTGGCCG400.006024914354.43552145
ACTAGGT400.006024914354.43552145
AAGCAGG41400.054.37097
GCAAAAG38750.049.8789633
AGCAGGT23400.049.7968868
AGCGTAC450.00959781848.38713145
Produced by FastQC (version 0.11.5)