FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_HBEpC_HIGH_12HPI_H3N2_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_HBEpC_HIGH_12HPI_H3N2_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772932
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG75390.42522781471596205No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG73570.4149623335807578No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA55790.31467647941376203No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC51450.29019725516827494No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC48550.2738401698429494No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG47030.2652668009827788No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC45540.25686264335011155No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT43680.2463715472449028No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA41710.23526000997218166No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA40200.2267430448545122No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT35570.2006281120764925No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG35370.19950003722647006No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG31880.17981513109357833No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG31120.17552844666349302No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC31060.17519002420848628No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG30940.1745131792984728No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG30610.17265185579593578No Hit
CTCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCC30580.1724826445684324No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT30510.17208781837092454No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA28000.15793047900314283No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT27980.15781767151814058No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA27740.15646398169811365No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT27600.15567432930309794No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG27480.15499748439308444No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT26970.1521208935255272No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA25740.14518323319788914No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC25740.14518323319788914No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT25290.14264506478533862No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT23680.1335640622426579No Hit
CTCCCAGCACAGGTCTCATAGGCAAATGGTGGCAACAACCAACCCATTAA23640.13333844727265343No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA23190.13080027886010293No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT22450.12662640191501986No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA22030.12425744472997272No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG21970.12391902227496598No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT21800.1229601586524469No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT21090.11895549293486722No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCA20840.11754539937233914No Hit
GCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGGT20830.11748899562983803No Hit
TCCTAGCTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA20720.11686855446232569No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA20700.11675574697732345No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG20630.11636092077981558No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGG20500.115627672127301No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA20500.115627672127301No Hit
TACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA20480.11551486464229875No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC20200.11393555985226732No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC19840.1119050251222269No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC19680.11100256524220896No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT19530.11015650910469212No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT19200.10829518560215506No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG18620.10502376853708997No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA18600.10491096105208772No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG18580.10479815356708548No Hit
TCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCT18480.10423411614207427No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC18440.10400850117206976No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT18420.10389569368706751No Hit
CTCATTGCCTGCACCATTCGCCTGGCCTGACTAGCAATTTCCATGGCCTC18160.10242919638203835No Hit
CTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGGTTG17990.10147033275951925No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC17820.10051146913700017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAAGG502.2530003E-887.09295145
AGGCTCG1550.084.2835145
TCACGCG353.419847E-582.94567145
GCAGGGG5650.067.876019
AGGGTGT6750.063.2501536
CCTAGCG350.003551898862.20925145
TAGCGCA702.3641405E-762.0337264
CAGTACG4300.057.2136089
GCAGGGT8150.056.8213049
GTGAGCC902.0336302E-856.449135145
CGGTCTG553.2027496E-452.78361145
AGCAGGG16800.051.686028
GTCGATT858.863226E-751.231148145
TTAGCGC859.0341564E-751.089483
ACAAGGT6900.050.349053
CGAAAGC6500.050.1069873
CGAGTCG450.009599769548.38497145
CGGCTCG752.5353303E-548.38497145
GGGTTAA3150.048.2716061
AAGGTAG6150.048.245735
Produced by FastQC (version 0.11.5)