FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_HBEpC_HIGH_12HPI_H1N1_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_HBEpC_HIGH_12HPI_H1N1_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645806
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT57900.35180331096131623No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA56500.3432968405753777No Hit
GGGCTAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC48120.29237953926525967No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC46240.2809565647469993No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA45320.2753665984933826No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC40990.24905730079972976No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT38680.2350216246629311No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT38660.2349001036574177No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG37370.22706199880180286No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC36840.22384169215569757No Hit
CCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCTTCTTGTATAGTTTA35930.21831248640483752No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT31890.19376524329112907No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACTTGGAGAAAAGAATAGAGA31730.19279307524702183No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC31510.19145634418637433No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACGTTCC31310.19024113413124025No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG31160.1893297265898897No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT31110.18902592407610616No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC30540.18556257541897406No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC29420.1787573991102232No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG29390.1785751176019531No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGCAG29260.17778523106611593No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC27810.16897495816639385No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC26940.16368879442656062No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC26820.16295966839348014No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC25700.1561544920847293No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT25460.1546962400185684No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG25370.15414939549375808No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT25320.15384559297997455No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAT25290.15366331147170445No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA25030.15208353840003014No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA24500.14886323175392482No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG24280.14752650069327733No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCTGCTGC23880.14509608058300918No Hit
GAACTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT23510.1428479419810111No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG23490.14272642097549773No Hit
GCACTGGAACTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG23400.14217957645068738No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG23350.14187577393690387No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC23160.14072132438452647No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT22560.13707569421912424No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG22380.13598200516950357No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC22340.13573896315847678No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA22290.13543516064469324No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA22180.13476679511436948No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGATAAAGC21900.1330655010371818No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC21120.12832618182215888No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC21050.12790085830286194No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA20580.12504511467329685No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT20510.12461979115399992No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT20470.12437674914297311No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT20320.12346534160162254No Hit
GCCTAGTCTGATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT20210.1227969760712988No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT19990.12146024501065132No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC19950.12121720299962449No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT19680.11957666942519349No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC19510.11854374087832953No Hit
GGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAAAGTC19340.11751081233146556No Hit
TTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC19070.11587027875703455No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCAGAGGCCATGG19060.11580951825427785No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA18820.11435126618811695No Hit
ATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGTGT18750.11392594266882003No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC18510.11246769060265913No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT17950.1090651024482837No Hit
ATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG17910.1088220604372569No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG17870.10857901842623006No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC17770.10797141339866302No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC17760.10791065289590632No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG17600.10693848485179905No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA17570.10675620334352895No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT17560.10669544284077224No Hit
CTATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGT17550.10663468233801554No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA17390.10566251429390827No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT17190.1044473042387742No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAG17120.10402198071947726No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC17020.10341437569191023No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG17000.10329285468639682No Hit
GATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGCCACC16990.10323209418364011No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT16940.10292829166985659No Hit
GATTAGTGGATTGGTGGTAGCAGCCATTTGTCTGTGAGACCGATGCTGTG16940.10292829166985659No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG16850.10238144714504625No Hit
GCTTAGTGACACCTCCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCT16850.10238144714504625No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTG16740.1017130816147225No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA16730.1016523211119658No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC16570.10068015306785853No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTA100.0071199113144.804672
GAGCGAA2050.0120.1293951
AGGCTCG1150.094.65074145
GCAGGTA13100.077.907929
GAGCAAA26800.074.593071
CTAACGC300.001929034872.56557145
GGATTAA809.822543E-1172.56556145
CGAAAGC5750.067.989043
TCTGACC1200.066.51843145
GCGAAAG6050.063.4268382
AGCGAGC2450.062.199055145
GGGCTCG350.003554161662.19905145
TAAATTA1300.061.401627145
AGCAAAA34450.057.58562
GCCACCG400.006029974754.42417145
CCGGAAG400.006086861254.295154
GTAGAAA7800.052.9303131
AAGCAGG43950.051.5535777
CACCGTC2300.050.357667
GTGTTTT9200.050.353079
Produced by FastQC (version 0.11.5)