FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_DARPA_DVG_P1_PB2_PLASMID.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_DARPA_DVG_P1_PB2_PLASMID.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882701
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT106250.5643487733846214No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA101020.53656953493943No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA92010.4887127589564142No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT91820.4877035705616558No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG80490.4275240731268534No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT74740.39698284539074447No Hit
GAACAGAGAAACTGACAATAACTTACTCATCGTCAATGATGTGGGAGATT70570.3748338158847315No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT70440.3741433185620021No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC66150.3513569069119313No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA60890.3234183229307256No Hit
TTATTGTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTC60400.3208156791758224No Hit
AACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGT60150.31948779970903507No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT60070.3190628782796631No Hit
GTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTGTA54290.2883623050075397No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC53850.286025237145994No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC49990.2655227781787974No Hit
GACAATAACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAAT49870.26488539603473943No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC49800.264513589784039No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA49170.2611673335277349No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG47290.251181679937494No Hit
GTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAA47100.2501724915427357No Hit
GTGAAACACAGGGAACAGAGAAACTGACAATAACTTACTCATCGTCAATG45940.2440111308168424No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT45800.2432675183154415No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT45250.24034618348850934No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT44700.23742484866157718No Hit
GTCTGATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCAT44330.23545958705073192No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA43390.2304667602556115No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC43050.22866084418078067No Hit
ATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGG42260.2244647450657327No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT42010.22313686559894533No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT41910.2226057138122304No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG39470.20964561021638595No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG38870.20645869949609633No Hit
GATTAATGGTCCTGAATCAGTGTTGGTCAATACCTATCAATGGATCATCA38120.20247506109573427No Hit
GTTTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCC38120.20247506109573427No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG37420.1987569985887297No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC35720.18972741821457578No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA35360.18781527178240198No Hit
TGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA33730.1791574976589485No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT32070.17034037799948054No Hit
GCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAA31800.1689062681753502No Hit
ATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAAGGCCA31670.1682157708526208No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA31380.16667543067114746No Hit
GAATCAGTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAAC31160.1655068967403746No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT31030.1648163994176452No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT30740.16327605923617186No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT30650.1627980226281284No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT30490.1619481797693845No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG29670.15759273511832203No Hit
TCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGTTGGTCAATACCTA29670.15759273511832203No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA29370.1559992797581772No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA29070.1544058243980324No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC28860.15329040564593105No Hit
ATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGGTCCCAG28510.15143137439242874No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG28180.14967857349626945No Hit
GTCCTGAATCAGTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGG28090.14920053688822602No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC27790.1476070815280812No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT27690.14707592974136627No Hit
GTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTGATGATCCATT27330.1451637833091925No Hit
GTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATT27250.14473886187982055No Hit
CCATAAAGTTTGTCCTTGCTCATTTCTCTCAGGAATCATTTCCGTTATCC27240.14468574670114903No Hit
ATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCTC27190.14442017080779157No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT26980.1433047520556902No Hit
GAAATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAG26960.1431985216983472No Hit
TGTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCT26640.1414988359808594No Hit
GTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCC26470.14059587794344403No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA26470.14059587794344403No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA26110.13868373151127023No Hit
CTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACTGACAATAACT26000.13809946454588382No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA25670.1363466636497245No Hit
GCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATATGG25610.13602797257769556No Hit
CTTTAGTACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACT25460.13523124489762314No Hit
CATCTACACCCATTTTGCTGATTCTCACTCCTCTCATTGACATCTCGATG25010.13284106185740593No Hit
CATTAATGCAGCGGATCCAGACATGATAAGATACATTGATGAGTTTGGAC24930.13241614042803398No Hit
GATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCT24780.13161941274796157No Hit
GGTCAGTGAAACACAGGGAACAGAGAAACTGACAATAACTTACTCATCGT24460.12991972703047378No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA24320.12917611452907288No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC24170.12837938684900047No Hit
TCCCTCATTTGTTGGAACAGAGTTCTTACAAACCCACTGTATTGGCCTCT24110.1280606957769715No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT24100.1280075805983No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT24020.12758265916892805No Hit
GAGTTCTTACAAACCCACTGTATTGGCCTCTAATGGCCTTAGGTACTAAA23810.12646724041682667No Hit
CTTACAAACCCACTGTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTG23730.12604231898745472No Hit
TTCCTATTCCACCATGTCACAGCCAGAGGTGATACCATCACTCGGTCTGA23630.1255111672007398No Hit
TTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGTTG23150.12296163862450808No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT23010.12221802612310717No Hit
ATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCCTCACA22950.12189933505107821No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC22950.12189933505107821No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA22700.12057145558429087No Hit
CTATCAGCTGAATCAATCTCCTGGTTGCTTTTCTGAGTATGGCTGTTGCT22570.11988095826156145No Hit
CCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGGTCC22210.11796881182938768No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT22200.11791569665071619No Hit
GAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATGATTCCTGAGA22020.1169596234346293No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA21990.11680027789861482No Hit
GAATTAGCTCATCCTTCAGTTTTGGTGGATTCACATTTAAGAGAACAAGC21840.11600355021854242No Hit
TTCTCACTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT21700.1152599377171415No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT21650.11499436182378402No Hit
TCCCAGAACCCTACAATGCTATACAATAAAATGGAATTTGAACCATTTCA21560.11451632521574057No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG21550.11446321003706908No Hit
TTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAG21500.1141976341437116No Hit
GTAAATGAGGAGAACTGCATTCTACTTTGCTTTGGTGGAGCGGCTGCGAA21280.11302910021293874No Hit
GCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACT21210.1126572939622383No Hit
TTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTCTACTT21090.11201991181818037No Hit
AGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTG21070.11191368146083738No Hit
CTGTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAA21050.1118074511034944No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC20850.11074514753006452No Hit
GTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGT20750.11021399574334959No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG20370.10819561895383283No Hit
GAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAA20370.10819561895383283No Hit
GGATTTATGTCAACTCTCCGACGTATTTTGACTTGGTTTCTAAAATGGAC20300.10782381270313236No Hit
CTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTT20240.1075051216311034No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA20220.10739889127376041No Hit
GTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTG20070.10660216359368802No Hit
GTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTACAAACCCACT20040.10644281805767353No Hit
ACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGTAT19990.10617724216431607No Hit
TCCTAATTCCACCAATCTGTGTGCTGTGGCACATCTCCAATAAAGATGCT19730.10479624751885723No Hit
CTGTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGT19720.10474313234018573No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGG19700.10463690198284274No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA19680.10453067162549975No Hit
GTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGA19590.10405263501745632No Hit
CTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCACCAAAACT19500.10357459840941287No Hit
CTTCTGAAAATGTCTTAAAAGTTGATGCATAGGATTCAATCGCTGATTCG19460.1033621376947269No Hit
GCATATGGGCATATGTTGCCAAACTCTAAACCAAATACTCATTCTGATGT19460.1033621376947269No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA19450.10330902251605541No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT19240.102193603763954No Hit
GCTTTAGCCTTTCGACTCTTTCAAAATAAGTTTTGTAGATTTTTGGATAA19110.1015031064412246No Hit
GCTTTTATCATACAATCCTCTTGTGAAAATACCATGGCCACAATTATTGC19010.10097195465450966No Hit
CCCACTGTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTT18880.10028145733178023No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA18870.10022834215310875No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAC100.0070714685145.13626145
TTTCGCG404.785761E-9108.852196145
CCGTATA607.2759576E-1296.75751145
TCGTATT406.085447E-790.71016145
GCCGACA259.353076E-487.08176145
CGCCGTT259.353076E-487.08176145
AATCGCG259.353076E-487.08176145
TACCGCC608.240022E-1084.66282145
GTATATA8400.084.487271
TATATAT8400.084.455842
GTACTTA651.553417E-978.15029145
TCTGCTT1300.078.15029145
GCTTGCG406.633768E-572.56813145
AGAGGTT903.6379788E-1272.56813145
GCTGCCC1500.072.56812145
ATATGAG10400.068.2016455
CGAATAA754.822141E-967.73025145
ACACATA3550.063.369354145
TCTGACC1400.062.201252145
CTAGTTT702.3205212E-762.201252145
Produced by FastQC (version 0.11.5)