FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_A549_LOW_3DPI_H3N2_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_A549_LOW_3DPI_H3N2_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2149993
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA39800.18511688177589417No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT38980.18130291586995864No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC37170.17288428380929613No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT35040.16297727480973193No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC30310.14097720318159176No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA28780.1338609009424682No Hit
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG26150.12162830297587014No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA24300.11302362379784492No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC22260.10353522081234684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6550.050.8643341
CCGACGC2100.048.384537145
CAGTACG1751.8189894E-1245.482799
CGAAAGC8100.042.8913763
GCGAAAG8750.038.054322
GCAAAAG38900.036.8408243
GAGCAAA45250.034.092421
TAGAAAC23350.032.8604242
GAGCGAA5550.031.3195231
CAAAAGC48200.030.4826474
ATACCGG1204.102234E-430.154496
AGCAAAA54300.026.9281022
TTAAACG1100.00975296126.3197083
AGGGTGT15350.025.9308996
CAAGGGT20050.023.8250184
AAGGGTG17850.023.516535
TGATGCG7800.023.1919869
GTATTAA10950.023.1499861
AAAAGCA67350.022.2441715
TTATAGT9650.021.7507554
Produced by FastQC (version 0.11.5)