FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_A549_LOW_3DPI_H3N2_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_A549_LOW_3DPI_H3N2_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2836412
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG87150.30725437630358354No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC86060.30341149311172005No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT78040.2751363342137884No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG70230.24760154730694975No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC60920.2147783890351613No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA60490.21326238924387572No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA59740.21061820356140082No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC56440.19898378655851126No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT55670.196269089257837No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT54700.19284927577516947No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC54020.19045188075639222No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA53750.18949997391070125No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG51960.1831891840818612No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT47000.16570230276842715No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT45300.15970881522148406No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA44310.15621849012061717No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA43470.15325700215624527No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA38150.1345009117152233No Hit
GTTCTTATCTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAA38070.13421886524242602No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA38050.13414835362422667No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG37480.13213877250554573No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT37040.13058751690516046No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG36040.12706193599519394No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT35980.12685040114059595No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC35780.1261452849586026No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC35230.12420621545812104No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG34950.12321905280333041No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC34760.12254919243043676No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC34570.12187933205754312No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA33780.11909412313866957No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG33610.11849477438397524No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC33300.11740184430188562No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG33080.11662621650169297No Hit
CTCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCC32930.11609737936519801No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG32870.11588584451060001No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT32050.11299486816442744No Hit
CCATTATATGATGCGATCAAGTGCATAAAAACATTCTTTGGATGGAAAGA32020.11288910073712845No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA30770.1084821245996703No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG29960.10562640406259738No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT29620.10442770655320877No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC28900.10188928829803286No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG151.2257264E-4145.1339145
AGGCTCG2000.079.82364145
GCAGGGT16950.058.9185269
GCTTATA2900.057.4231262
CGAAAGC10550.056.262473
AGCGAAA10400.054.3310621
CAGTACG3650.053.5319949
AGGGTGT15300.052.037976
CCTAGCG701.687539E-551.833534145
AGCGTAC450.00960585548.377968145
GTCGCAA604.924163E-448.377964145
AAGGTAG7600.047.620165
GCAGGGG6100.047.4539839
TAAACTA2700.045.5742535
CGGCTCG657.305579E-444.65658145
TAGGCAT3600.044.224489
ACAAGGT9750.043.8030933
TAGAAAC23250.042.9747282
TAGGACC12750.042.569019
TTTAAAC2900.042.433453
Produced by FastQC (version 0.11.5)