FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_A549_LOW_12HPI_PR8_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_A549_LOW_12HPI_PR8_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232752
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA41500.3366451646397653No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG37780.30646877879735746No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT37280.3024128129583241No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT33700.27337209755084557No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA33030.2679371033265409No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA27380.22210468934546446No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT26580.21561514400301116No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG25810.20936895661089983No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA25730.2087200020766545No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA23610.19152270691915324No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA22910.1858443547445066No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG22770.18470868430957726No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT22450.18211286617259595No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC21460.17408205381130998No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT19790.1605351279089387No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG17600.14276999753397276No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG17330.14057977598089477No Hit
GCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAG17090.13863291237815878No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG16950.13749724194322946No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG16940.13741612262644878No Hit
GTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGCCTGACTAGCAAC16850.13668604877542279No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT16660.13514478175659012No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA16430.13327903747063483No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA16350.13263008293638948No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT16140.13092657728399548No Hit
GACTTGAAGATGTCTTTGCAGGGAAGAACACTGATCTTGAGGTTCTCATG15940.12930419094838216No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA15900.1289797136812595No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG15840.1284929977805755No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA15820.12833075914701417No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT15810.1282496398302335No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG15630.1267894921281815No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA15490.12565382169325215No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT15470.12549158305969085No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG15450.1253293444261295No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA15370.12468038989188418No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG14930.12111113995353485No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT14780.11989435020182486No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT14750.11965099225148285No Hit
GAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAGACCAA14740.1195698729347022No Hit
GTTCACAGGTTGCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCC14520.11778524796552754No Hit
CCTTTCAGTCCGTATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACG14450.11721741274806287No Hit
CTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTATC14410.11689293548094021No Hit
TGTCTAGCCTGACTAGCAACCTCCATGGCCTCTGCTGCTTGCTCACTCGA14220.11535166846210754No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC14160.11486495256142354No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC14080.11421599802717822No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA13960.11324256622581022No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA13920.11291808895868756No Hit
GTCAAGTGCAAGATCCCAATGATATTTGCGGCAATAGCGAGAGGATCACT13910.11283696964190688No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT13860.11243137305800355No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT13840.11226913442444221No Hit
GGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCA13800.11194465715731955No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT13770.11170129920697756No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC13580.1101600321881449No Hit
CAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGTATGTG13470.10926771970355756No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG13300.10788869131828625No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA13190.10699637883369892No Hit
CCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAAC12820.10399496411281427No Hit
GGCTAGACAAATGGTGCAAGCGATGAGAACCATTGGGACTCATCCTAGCT12730.10326489026178826No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG12660.1026970550443236No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC12600.1022103391436396No Hit
GCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGGGATTTTAGGAT12420.1007501914415876No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG12410.10066907212480693No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC12370.10034459485768427No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2800.087.9316941
GAGCGAA1300.083.5550161
GCTCACG353.4196833E-582.94444145
GCAAAAG14100.081.6156543
TAGGCCG451.2253568E-680.640434145
AGCAAAA15950.073.068662
ATGTGCG451.187761E-464.512344145
TGACGCG350.0035518562.208332145
ACGGCCG702.3180291E-762.208332145
TTAGTAG350.00358344362.069431
GCAGGGG6300.059.7293439
CCCCCCG501.9999893E-458.061108145
ACAAGGG3550.057.0854073
GCACACC1550.056.188168145
CAAGGGT3750.054.040864
CAAAAGC22550.052.63724
AGGGTGT2900.052.4103166
AAGCAGG24300.051.5270467
GCAGGGT6450.050.4867949
AGCAGGG14050.050.474818
Produced by FastQC (version 0.11.5)