FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_A549_LOW_12HPI_H3N2_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_A549_LOW_12HPI_H3N2_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478554
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG56380.3813185044306802No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG47500.3212598254781361No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC43840.29650591050445235No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA43310.292921327188591No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC42140.2850081904347085No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT39180.2649886307838605No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC36930.24977106010331718No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG35690.24138448781715108No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT33850.22893989668284015No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA29880.20208933863761486No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA29710.20093956663064047No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA26570.17970260132534896No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG25720.17395374129047705No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC24450.16536426806190374No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC24360.164755565234682No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT24020.16245602122073324No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG23940.16191495204098058No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG23720.1604270117966608No Hit
CTCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCC22930.15508395364660338No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA22810.1542723498769744No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG21850.1477795197199426No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT21820.14757661877753536No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG21340.14433020369901944No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT21340.14433020369901944No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT20720.1401369175559364No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA19540.13215614715458482No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG19490.13181797891723943No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT18980.12836866289631627No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG18780.12701598994693464No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT18760.12688072265199649No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA18290.12370194122094966No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT18250.12343140663107334No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT18080.12228163462409895No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA17960.12147003085446997No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG17410.11775018024367051No Hit
TCCTAGCTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA17180.11619460635188164No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA16700.11294819127336574No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT16660.1126776566834894No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC16420.11105444914423146No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC16230.10976940984231891No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC15570.10530558910935954No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC15500.10483215357707598No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC15380.10402054980744702No Hit
GCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGGT15290.10341184698022528No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCA15220.1029384114479417No Hit
TACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA15070.10192390673590548No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGG14930.10097703567133835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG100.0070710583145.13802145
AGGCTCG850.0128.06297145
TCTACCG301.592172E-596.75869145
CCTAGCG406.084356E-790.711266145
TTAAACG259.352222E-487.08282145
ACAGACG607.9815436E-872.569016145
TAGTGCG406.632896E-572.56901145
TTAGCGC406.715796E-572.387313
CGAGTCG451.18843716E-464.50578145
TAGCGCA451.2034748E-464.3420944
AGGGTGT6150.060.020386
GCAGGGT9350.058.0489779
ACCTCGC400.00602871954.426758145
CGAAAGC5350.054.1213533
AGCGAAA5750.049.120751
TTTAGCG604.981443E-448.2631032
CAGGGTG7000.045.49298
GATATAG803.7700003E-545.2420653
GCAGGGG4200.044.799079
ACAAGGT6200.044.358918
Produced by FastQC (version 0.11.5)