FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_A549_HIGH_6HPI_H3N2_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_A549_HIGH_6HPI_H3N2_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177071
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC41260.3505311064498233No Hit
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG38750.32920698921305513No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT34010.2889375407260904No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG32490.2760241310847009No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC27690.23524494274347085No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA27260.2315918071212357No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA25540.2169792646322949No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC25520.21680935134753979No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC24690.2097579500302021No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA24260.2061048144079669No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT22920.1947206243293735No Hit
GCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGATGCA22790.1936161879784652No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG22360.18996305235622998No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT22300.18945331250196462No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA22270.18919844257483193No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT21590.18342139089315768No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT19690.16727962884142078No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA19240.16345657993443047No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA18780.1595485743850626No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC18440.15666004854422547No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG17810.151307780074439No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGATGCATTGAGCAAA16940.14391655218759106No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT16490.14009350328060075No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC16290.13839437043304947No Hit
GTCCCTAAGAGCCTGAACAACAAGAAGCAGTTTTCTTGACTCCGCTGAAA16110.13686515087025336No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG16110.13686515087025336No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG16080.1366102809431207No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC16010.13601558444647774No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC15870.13482619145319186No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT14970.12718009363921123No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC14950.1270101803544561No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG14760.1253960041492824No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC14700.12488626429501704No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC14620.12420661115599654No Hit
CTCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCC14530.12344200137459847No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA14370.12208269509655746No Hit
GTTCTTATCTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAA14060.11944903918285302No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT13830.11749503640816909No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA13830.11749503640816909No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG13710.11647555669963834No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG13630.11579590356061785No Hit
GTATGGAGAGGATCTGAAAATTGAAACAAACAAATTTGCAGCAATATGCA13390.11375694414355635No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA12760.10840467567376989No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG12570.1067904994685962No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA12570.1067904994685962No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT12470.10594093304482058No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT11980.10177805756832001No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG11960.10160814428356489No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTCG950.091.672386145
CCGGTAC259.462113E-486.826013
CCGACGC1200.078.6218145
TAGTGCG651.3884346E-766.99136145
GTCAAGG451.18793425E-464.5102145
TAAACTA2400.060.288155
CAGTACG5350.059.4917539
GCTCACG502.0002807E-458.05918145
GCTTATA1750.057.8963052
GAGTCGC651.0871745E-555.826134145
ATTGCCG651.0871745E-555.826134145
GCAGGGT8700.055.7074669
TTTAAAC2600.055.65773
AGCGAAA5300.054.6540031
TAGAAAC10450.053.3255422
CGAAAGC5600.052.9742053
GCAGGGG2600.052.8613369
TTATATT4850.052.2260482
TATATTC5150.050.578263
AGGGTGT6600.049.3224756
Produced by FastQC (version 0.11.5)