FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_A549_HIGH_1DPI_H1N1_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_A549_HIGH_1DPI_H1N1_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937619
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG752.5746105E-548.259595
GAGCAAA33250.047.260681
GCGAAAG8650.046.0314982
TCAACGC1202.002198E-742.2173278
CGAAAGC10150.039.9410363
AGCGAAA10100.039.4342161
TGACCGG3200.038.444959
GCAAAAG39850.037.786123
GAGCGAA7450.031.1046711
GCCGACC2601.09139364E-1030.69841145
TAGCGCA4800.030.1528999
CAAAAGC55000.028.0351584
ATAGCGC5000.027.5015748
AGCAAAA56650.026.8366242
GATAGCG5100.025.5465567
TAGGACG1500.001507900724.1304194
ATGACCG8100.024.124198
AAGCAGG72450.023.7776327
AAAGCAG76450.023.0075326
CAACGCA2603.2582557E-622.2667589
Produced by FastQC (version 0.11.5)