FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n02_A549_HIGH_12HPI_PR8_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n02_A549_HIGH_12HPI_PR8_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640480
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA64880.39549400175558375No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG59880.36501511752657756No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT45170.2753462401248415No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG42300.257851360577392No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA39120.23846679020774406No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT38890.23706476153320977No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA32210.1963449722032576No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG31630.19280942163269288No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG31000.1889690822198381No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT30860.18811567346142594No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT30690.1870793913976397No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA29150.17769189505510583No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT27940.17031600507168634No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA27920.17019408953477033No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT26680.16263532624597676No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA25910.15794157807470985No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA25670.15647859163171754No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA25580.15592997171559542No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT25220.153735492051107No Hit
GAGTTATGGGCTGGCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGG24280.14800546181605384No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC23890.14562810884619137No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG23270.1418487272017946No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG22980.14008095191651224No Hit
GTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGCCTGACTAGCAAC22090.13465571052374914No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA21980.133985175070711No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA21890.13343655515458888No Hit
GCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAG21810.1329488930069248No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT21610.13172973763776458No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT21190.12916951136252805No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG21090.1285599336779479No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA19640.11972105725153613No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT19150.11673412659709353No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG19050.1161245489125134No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC18720.114112942553399No Hit
GAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAGACCAA18490.11271091387886473No Hit
GCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGTACAATTCAAG18470.1125889983419487No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG18440.11240612503657466No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA18370.11197942065736859No Hit
GACTTGAAGATGTCTTTGCAGGGAAGAACACTGATCTTGAGGTTCTCATG18130.11051643421437628No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT17960.10948015215059008No Hit
GCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCCTGTT17740.10813908124451381No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG17590.10722471471764361No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT17370.10588364381156734No Hit
GGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCA17190.10478640397932314No Hit
GTCTGGAACTGTGACTTTTGGTCCTTCCTATCCAAACACCATTACCATAC16960.10338437530478883No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA16420.10009265580805618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2350.092.4720841
GCAAAAG17850.081.119233
GCAGGGG10650.068.6474469
TGACGCG350.003553433362.202263145
AGCAAAA25800.057.8068852
ACAAGGG2650.057.3727233
CCCCACG1700.055.494175145
AAGCAGG30700.054.4710777
GTCTATA400.00602874154.42698145
AGTTATG7350.054.1760642
AGCAGGG19900.052.381188
CAAAAGC28050.052.1359564
AGCGAAA4550.050.944331
GCAGGGT6150.049.434129
CAAGGGT3150.048.264474
CTACTAA450.00969701248.261536
AAGGGTG2950.046.626975
GAGTTAT9300.045.175431
AGGGTGT3050.045.096846
AATACTA2250.045.0482182
Produced by FastQC (version 0.11.5)