FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13397492
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTCG6500.046.791443145
ACAAGGG41650.041.261383
CTATGCT40200.041.126264
TCTATGC39250.041.0132063
GCAAAAG167750.040.5030253
GCAGGGT49150.040.307219
GAGCGAA17250.039.637121
AGCAGGG88500.035.2934888
ACGGCCG5150.033.74695145
GGTAGTT21350.033.5267457
AGGGTGA35350.033.1345679
CAAGGGT53650.033.113634
AAGGGTG44950.032.7469025
CAGGGTG35850.032.470648
CGAAAGC46100.030.9867273
GAGCAAA223500.030.8853131
GCAGGTA110550.030.7393599
AGCAAAA233250.030.1602752
CTCTATG77200.029.9680562
CAGTACG8000.028.0173729
Produced by FastQC (version 0.11.5)