FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_INFANT_Exp2_Shed_Ind2_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_INFANT_Exp2_Shed_Ind2_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361010
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC53990.3966906929412715No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT42050.3089617269527777No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA38110.2800126376734925No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT36860.2708282819376786No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA36540.2684770868693103No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA31220.22938846885768657No Hit
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG29610.21755901866995836No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA24820.1823645674903197No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA24430.17949904850074577No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT23730.17435580928869002No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA20980.15415022666989955No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA20870.15334200336514794No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA19940.14650884269770245No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC18730.13761838634543463No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG18320.1346059176640877No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG17700.13005047721912402No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC17360.12755233245898265No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT17090.1255685116200469No Hit
GTAGTAAACAGTATTTGCAACACTACAGGAGATATGCTTATAAGAAGTGC16580.12182129447983482No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT16380.12035179756210461No Hit
TCTCTGTACTGAGAACGTAGGTTGTATGCTGATTTGGCCCGCAGATGCCC15920.11697195465132512No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG15780.11594330680891396No Hit
CTACAGGAGATATGCTTATAAGAAGTGCCATAGGCCAGGTTTCAAGGCCC15560.11432686019941074No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA15170.11146134120983682No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA14990.11013879398387962No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT14710.1080814982990573No Hit
CTCAATATGAGTGCAGACCGTGCTAGAAAAGTGAGATCTTCGAACTCAGC14530.10675895107310011No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA14490.10646505168955409No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC14380.10565682838480246No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT13770.1011748627857253No Hit
CCTTTGGGGGACTCTCCAATGGGCCATGTTTCTGATTTGTTCTCAAAGAA13700.10066053886451973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1900.0103.057211
AGACGCG501.9645086E-10101.48941145
CTACTAA301.5972877E-596.6956566
CTTCCCG554.1654857E-1092.2631145
GCCCACC703.092282E-1182.848495145
AGCGAAA3800.080.155611
AGAGTTA554.398862E-879.08266145
AGCAGGC7350.077.914188
GGCAGCC1600.063.430878145
GCAAAAG30300.062.7085723
TACTGCG350.003568255362.13637145
GCAGGGT10150.060.7057159
TTAGTAG606.7948185E-660.4347842
TTTAAAC2450.059.201423
CGAAAGC5300.057.470063
ATTAGTA651.0919146E-555.7859541
TAAACTA2600.055.7859545
ACAAGGG4050.055.5104643
GAGCAAA35050.055.4517141
CAAAAGC34950.054.5728724
Produced by FastQC (version 0.11.5)