FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_INFANT_Exp2_Shed_Cnt4_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_INFANT_Exp2_Shed_Cnt4_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765516
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC64910.3676545553821092No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA41180.233246257751275No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA40430.22899820788936492No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA36940.2092306158652768No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA27730.15706456356102125No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA22840.12936727846136767No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT22240.1259688385718396No Hit
AATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCA20390.11549031557912814No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA19380.10976960843175593No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC18450.10450202660298746No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC18060.10229304067479422No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT18010.10200983735066688No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTT350.003564441662.153924
TCGTACT1900.049.61552
TACTGAT19100.048.2017337
GGTACTG21450.045.299445
CTATTCG5400.044.300899
AGCGAAA5600.044.026941
GTACTGA27250.040.9797676
ACTGATC25500.040.084028
ATTAACG1105.2601627E-639.5524943
CGAAAGC6850.037.0503923
GCCGAAT1209.574906E-636.2461859
AGCAGGT27600.035.9947431
TAACACG850.002740159434.123724
AACGCCG1352.1405189E-532.2279556
TCTCCGC1801.2939017E-732.220657145
ATGTTCG1402.7498345E-531.068167
CTGATCC33100.029.7853839
CAGGTAC33650.029.7378353
GTATATA13350.029.3318941
TTAACGC1504.3952525E-529.0051634
Produced by FastQC (version 0.11.5)