FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_HBEpC_LOW_3DPI_H3N2_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_HBEpC_LOW_3DPI_H3N2_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015325
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG112640.5589172962177316No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC86550.4294592683562205No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA79100.3924925260193765No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG79070.3923436666542617No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT76650.3803356778683339No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC75100.37264461067073545No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG68600.34039174822919377No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG65490.32495999404562537No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA57700.286306178904147No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA57220.2839244290623101No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG51550.2557900090556114No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG51170.2539044570974905No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT51150.2538052175207473No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC49030.24328582238596752No Hit
CTCCCAGCACAGGTCTCATAGGCAAATGGTGGCAACAACCAACCCATTAA47940.23787726545346283No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG46110.22879684418145957No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA45750.22701053180008185No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC44920.22289208936523888No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT44120.21892250629551063No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT40700.20195253867242255No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT40400.20046394502127451No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG40270.19981888777244367No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT39940.19818143475618077No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCA36720.18220386290052473No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG36050.17887933707962736No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT35910.17818466004242492No Hit
TCCTAGCTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA34600.17168446776574497No Hit
GCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGGT34210.16974929601925248No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA32820.16285214543559973No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA32150.15952761961470233No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA32030.15893218215424312No Hit
TACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA31590.1567489114658926No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT30990.15377172416359644No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC30390.1507945368613003No Hit
CTCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCC29970.14871050574969297No Hit
CTCATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT29790.14781734955900414No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG29630.14702343294505849No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC29350.14563407887065363No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGG29290.145336360140424No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA28190.13987818341954772No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT28080.1393323657474601No Hit
GTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCCATTGAGGGCA27920.13853844913351443No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA27660.1372483346358528No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC27580.13685137632887995No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA27260.13526354310098868No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT27120.13456886606378624No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA27000.133973428603327No Hit
GACTAAGGGGATTTTGGGGTTTGTGTTCACGCTCACCGTGCCCAGTGAGC26900.13347723071961098No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC26750.13273293389403695No Hit
CTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGGTTG26180.1299046059568556No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACCGTCGT25890.12846563209407913No Hit
ATCCCAATGATACTCGCGGCAACAACAAGCGGGTCACTTGAATCGTTGCA25660.12732437696153226No Hit
GTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATAGGCAA25630.12717551759641746No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG24610.122114299182514No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA24450.12132038256856834No Hit
GCACATACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATT23740.11779737759418456No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGGGT23350.11586220584769205No Hit
CCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATGTCTTTGCTGGGAAAA23320.11571334648257725No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGATCCAAATAACATGGACAAAGC23290.11556448711746244No Hit
GGCAATGAGAGCCGTTGGGACTCATCCTAGCTCCAGTACTGGTCTAAGAG23190.11506828923374642No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA22820.11323235706399712No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACC22720.11273615918028108No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACC22470.11149566447099103No Hit
CCACAATATCAAGTGCAAGATCCCAATGATACTCGCGGCAACAACAAGCG22430.11129718531750461No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG22400.11114832595238981No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG22220.11025516976170098No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGG21810.10822075843846526No Hit
TGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA21760.10797265949660725No Hit
TCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCT21710.10772456055474923No Hit
ATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTGCA21660.10747646161289122No Hit
CTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG21520.10678178457568879No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT21510.10673216478731717No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG21360.10598786796174313No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT21050.10444965452222346No Hit
AGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCAGCGGAGG20680.10261372235247415No Hit
ATCAAGTGCAAGATCCCAATGATACTCGCGGCAACAACAAGCGGGTCACT20670.10256410256410256No Hit
GTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAATG20470.10157170679667052No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT20310.10077779018272488No Hit
GACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGGTTTGTGTTCACG20250.10048007145249525No Hit
GTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAA20220.10033121208738044No Hit
CCTCTGACTAAGGGGATTTTGGGGTTTGTGTTCACGCTCACCGTGCCCAG20200.10023197251063726No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGTC100.0070892856145.014423
TCACGCG301.1021257E-7120.83336145
CCTAGCG501.8189894E-12116.000015145
AGGCTCG1350.0112.7778145
TTTAGCG800.099.697412
CCCACGC651.6370905E-1189.23078145
GTCAAGG651.6370905E-1189.23078145
TTAGCGC900.088.619933
GTGAGCC801.8189894E-1281.56251145
TAGCGCA1050.075.959944
CCGATAG406.661547E-572.507214
TAGTGCG300.001936026872.500015145
AGCGAAA7500.066.7066351
CGAAAGC7700.064.9743
ATTTAGC1400.062.1490331
GTCTAGT350.003567026562.142868145
AGGGTGT6900.059.897266
TCTCCGC1600.058.906258145
CTTATAA1550.056.129044145
ACAAGGT8850.055.7117543
Produced by FastQC (version 0.11.5)