FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_HBEpC_LOW_2DPI_H3N2_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_HBEpC_LOW_2DPI_H3N2_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930661
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG91320.4729986258592264No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC73330.3798181037478874No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA73060.3784196189802353No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG72540.3757262409092016No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC68760.35614745416207194No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT67140.34775654555615926No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG57740.29906855734901155No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA52160.2701665388175345No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA51590.2672141820858245No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG47420.24561536178541962No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT45770.23706906598310115No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG43630.2259847793061547No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG43560.22562220918120787No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG43200.2237575628243384No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC42730.221323163413981No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA41190.21334662066515042No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT39330.20371261448799144No Hit
CTCCCAGCACAGGTCTCATAGGCAAATGGTGGCAACAACCAACCCATTAA39230.20319465716663881No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT38280.19827406261378874No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT38120.19744533089962454No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA35770.18527333384783762No Hit
CAAAAGCACTCAAGCAGCAATCAACCAAATCAATGGGAAGCTGAATAGGT35690.18485896799075552No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT35020.18138865393769282No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG34370.1780219313489007No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC33060.1712366904391812No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCA33020.17102950751064014No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA32300.16730021479690116No Hit
TCCTAGCTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA32110.16631609588633114No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT31600.16367451354743273No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA31220.1617062757262927No Hit
GCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGGT30770.15937546778020584No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT30350.15720004703052476No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG29190.1511917421028342No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC28570.14798040671044788No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC28510.14766963231763627No Hit
TACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA28200.14606396462144314No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGG27830.14414752253243837No Hit
CTCATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT26210.1357566139265257No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG26130.1353422480694436No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT25390.13150936389143408No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT25180.13042165351659354No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG25050.1297483089988351No Hit
GACTAAGGGGATTTTGGGGTTTGTGTTCACGCTCACCGTGCCCAGTGAGC24250.12560465042801403No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA24240.12555285469587876No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA24220.12544926323160824No Hit
GTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCCATTGAGGGCA24160.12513848883879666No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC23960.12410257419609139No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA23850.12353282114260349No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT23020.11923377537537662No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA22800.11809426926840082No Hit
GTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATAGGCAA22720.11767990341131872No Hit
CTCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCC22710.11762810767918346No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACCGTCGT22410.11607423571512554No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA22220.11509011680455553No Hit
ATCCCAATGATACTCGCGGCAACAACAAGCGGGTCACTTGAATCGTTGCA21730.11255212592992762No Hit
CTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGGTTG21720.11250033019779236No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA21360.11063568384092287No Hit
GCACATACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATT21180.10970336066248812No Hit
GTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAATG21110.10934079053754128No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACC21030.10892642468045918No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC20190.10457558318109704No Hit
GGCAATGAGAGCCGTTGGGACTCATCCTAGCTCCAGTACTGGTCTAAGAG20120.1042130130561502No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA20060.10390223866333863No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACC20010.10364326000266232No Hit
ATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTGCA19980.1034878728062565No Hit
CCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATGTCTTTGCTGGGAAAA19960.10338428134198598No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGATCCAAATAACATGGACAAAGC19790.1025037538956865No Hit
CCACAATATCAAGTGCAAGATCCCAATGATACTCGCGGCAACAACAAGCG19590.10146783925298122No Hit
CTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG19540.10120886059230491No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG19380.10038012887814068No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCG100.0070889764145.016371
CGGCTCG151.230009E-4145.00511145
TACCGGA203.8656686E-4108.756642
CGTCACC301.5995589E-596.670075
CCCACGC651.6370905E-1189.23391145
CAGTACG1400.088.020579
CCTAGCG259.386769E-487.00307145
GTGGGGC950.083.95032145
AGGCTCG2200.082.38927145
TATAGTG2150.077.560875
GATATAG2400.069.481613
CCACGAC554.062187E-665.911415145
TCCGTCA451.1939309E-464.446713
CCTAACC350.00356650962.1450425
GTCCGGC350.00356650962.145042145
GCAGGGG5350.060.970829
GCCCACC1800.060.418793145
CGAAAGC6150.060.124073
CATAATA4550.058.959652
ATAAGCC502.0103654E-458.002045145
Produced by FastQC (version 0.11.5)